[1]钟世顺,张振书,王继德,等.双歧杆菌粘附素对脂多糖和H2O2调节肠上皮细胞增殖和凋亡的影响[J].南方医科大学学报,2004,(03):264-268.
 ZHONG Shi-shun,ZHANG Zhen-shu,WANG Ji-de,et al.Effect of Bifidobacterial adhesin on lipopolysaccharide-and H2O2-induced proliferation and apoptosis of intestinal epithelial cells in vitro[J].Journal of Southern Medical University,2004,(03):264-268.
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双歧杆菌粘附素对脂多糖和H2O2调节肠上皮细胞增殖和凋亡的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2004年03期
页码:
264-268
栏目:
出版日期:
2004-03-01

文章信息/Info

Title:
Effect of Bifidobacterial adhesin on lipopolysaccharide-and H2O2-induced proliferation and apoptosis of intestinal epithelial cells in vitro
作者:
钟世顺 张振书 王继德 赖卓胜 潘令嘉
第一军医大学南方医院全军消化内科研究所, 广东, 广州, 510515
Author(s):
ZHONG Shi-shun ZHANG Zhen-shu WANG Ji-de LAI Zhuo-sheng PAN Ling-jia
Institute for Digestive Diseases of PLA, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China
关键词:
双歧杆菌粘附素脂多糖过氧化氢上皮细胞/代谢细胞增殖/药物作用细胞凋亡/药物作用
Keywords:
Bifidobacterialadhesinliposaccharidehydrogen peroxideepithelial cellmetabolism metabolismcell proliferation/drug effectscell apoptosis/drug effects
分类号:
R378.2
摘要:
目的 研究双歧杆菌分泌型粘附素对脂多糖(LPS)和H2O2体外调节肠上皮细胞增殖和凋亡的影响。方法3H-TdR掺入法、流式细胞仪和荧光染色等方法观察肠上皮细胞增殖和凋亡的情况。结果 100 μg/L LPS对细胞增殖和凋亡均有促进作用,200 μmol/L H2O2可抑制肠上皮细胞增殖,促进凋亡。丫啶橙荧光染色见细胞核染色质致密浓染或裂解成碎片。预先经双歧杆菌分泌型粘附素处理后,LPS组细胞增殖和凋亡率均显著下降,H2O2组细胞凋亡明显减少。结论 在体外,双歧杆菌分泌型粘附素能抑制LPS和H2O2对肠上皮细胞的损害作用,维持肠上皮细胞增殖与凋亡的平衡。
Abstract:
Objective To study the effect of Bifidobacterial adhesin on proliferation and apoptosis of intestinal epithelial cell induced by lypopolysaccharide (LPS) and H2O2 in vitro. Methods With 3H-TdR incorporation method, flow cytometry and fluorochrome staining, the proliferation and apoptosis of intestinal epithelial cells induced by LPS and H2O2 in vitro were studied. Results LPS at the dose of 100 μg/L effectively stimulated the proliferation and apoptosis of cells, whereas H2O2 at the dose of 200 μmol/L obviously restrained the proliferative ability while enhanced the apoptosis of the cells. Fluorochrome staining showed cell shrinkage, nuclear condensation and fragmentation under microscope. After treatment with Bifidobacterial adhesin, the cell proliferation and apoptosis decreased significantly in LPS group, and in H2O2 group, cell apoptosis was signifi- cantly decreased. Conclusions Bifidobacterial adhesin can protect intestinal epithelial cells from the damage by LPS and H2O2, and maintain the balance between the proliferation and apoptosis of the cells.

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备注/Memo

备注/Memo:
收稿日期:2003-12-7。
基金项目:广东省自然科学基金资助(010621)
作者简介:钟世顺(1969- ),男,第一军医大学在读博士研究生,主治医师,电话:020-61641544,E-mail:zss@fimmu.com
更新日期/Last Update: 1900-01-01