[1]吴辉绪,陈清,李建栋,等.口服霍乱疫苗O139rBS-WC后肠道菌群的改变[J].南方医科大学学报,2004,(02):220-222,225.
 WU Hui-xu,CHEN Qing,LI Jian-dong,et al.Dynamics of intestinal flora after oral vaccination with inactivated whole-cell/recombinant B subunit O139 cholera vaccine[J].,2004,(02):220-222,225.
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口服霍乱疫苗O139rBS-WC后肠道菌群的改变()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2004年02期
页码:
220-222,225
栏目:
出版日期:
2004-02-01

文章信息/Info

Title:
Dynamics of intestinal flora after oral vaccination with inactivated whole-cell/recombinant B subunit O139 cholera vaccine
作者:
吴辉绪1 陈清1 李建栋1 黄秀芬2
1. 第一军医大学流行病学教研室, 广东, 广州, 510515;
2. 广州医学院第一附属医院, 广东, 广州, 510120
Author(s):
WU Hui-xu1 CHEN Qing1 LI Jian-dong1 HUANG Xiu-fen2
1. Department of Epidemiology, First Military Medical University, Guangzhou 510515, China;
2. First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China
关键词:
霍乱/免疫学疫苗/治疗应用肠道菌群
Keywords:
cholera/immunologyvaccines/therapeutic useintestinal flora
分类号:
R516.5;R979.5
摘要:
目的 观察国产冻干O139rBS-WC口服霍乱疫苗对健康受试者肠道菌群的影响。方法 利用直接快速涂片法分别于服苗前、服苗后2周末、服苗后3个月末收集受试者的粪便,检查粪便的细菌分布特点。结果 共检查了458份粪便标本,服苗后细菌总数、G+b、G+c、G-c呈下降趋势,而G-b呈显著的增高趋势;口服疫苗后各免疫组之间及与安慰剂组比较,以G+b及G-c变化明显,其中0、14、28 d各服1粒O139rBS-WC 疫苗的免疫组与其他各组间的差异较大;各免疫组在不同采样时间,肠道菌群值有不同的改变,以G-b表现明显。不同性别的受试者在服苗后,G+b、G-b、G-c占总菌群数比值的改变有统计差异。结论 冻干口服O139霍乱rBS-WC疫苗对受试者的肠道菌群有一定的影响。
Abstract:
Objective To investigate the change of intestinal flora in subjects receiving oral vaccination with inactivated whole-cell/recombinant B subunit (WC/rBS) O139 cholera vaccine made in China. Methods The fecal smears of 5 groups of subjects receiving the vaccine were collected before and 2 and 3 months after the vaccination to examine the distribution of the intestinal flora. Results In the 458 specimens examined, the total amount of bacteria and percentages of G+b, G+c and G-c were decreased whereas the amount of G-b significantly increased after vaccination. Compared with the placebo control group, the vaccinated groups exhibited significant changes in G+b and G-c, which also varied significantly between the group receiving 3 doses of WC/rBS vaccine and the other vaccinated groups. The vaccination produced distinct changes in the percentage of the intestinal flora at different time points, showing the most obvious effect on G-b. Oral vaccination was shown to affect the percentage of the intestinal flora very likely through its effect on the amount of G-b. The percentages of G+b, G-b and G-c varied significantly between male and female subjects after vaccination. Conclusion Oral vaccination with WC/rBS affects the intestinal flora in human, and the mechanism as well as the implications needs to be further explored.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2003-7-6。
作者简介:吴辉绪(1966-),男,副主任医师,第一军医大学在读硕士研究生,现工作单位为广州军区卫生防疫队,电话:020-87797077,E-mail:hunjeny-wu@mail.china.com
通讯作者:陈清,教授,电话:020-62648312,E-mail:qingchen@fimmu.com
更新日期/Last Update: 1900-01-01