[1]姜耀东,郑少斌,王战会.RT-PCR法检测肾癌组织中MN/CAⅨ基因表达[J].南方医科大学学报,2004,(01):99-101.
 JIANG Yao-dong,ZHENG Shao-bin,WANG Zhan-hui.Detection of carbonic anhydrase Ⅸ gene expression in renal cell carcinoma with reverse transcription-PCR[J].Journal of Southern Medical University,2004,(01):99-101.
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RT-PCR法检测肾癌组织中MN/CAⅨ基因表达()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2004年01期
页码:
99-101
栏目:
出版日期:
2004-01-01

文章信息/Info

Title:
Detection of carbonic anhydrase Ⅸ gene expression in renal cell carcinoma with reverse transcription-PCR
作者:
姜耀东1 郑少斌1 王战会2
1. 第一军医大学南方医院, 泌尿外科, 广东, 广州, 510515;
2. 第一军医大学南方医院, 感染内科实验室, 广东, 广州, 510515
Author(s):
JIANG Yao-dong1 ZHENG Shao-bin1 WANG Zhan-hui2
1. Department of Urology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China;
2. Laboratory of Department of Infectious Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China
关键词:
碳酸酐酶Ⅸ(MN/CAⅨ)基因表达肾癌/诊断聚合酶链反应
Keywords:
carb onic anhydrase Ⅸ(MN/CAⅨ)gene expressionrenal cell carcinoma/diagnosispol ymerase chain reaction
分类号:
R730.53
摘要:
目的 评价RT-PCR法检测碳酸酐酶Ⅸ(MN/CAⅨ)基因在肾癌组织的表达的可靠性。方法 以5例肾癌和5例正常肾组织为研究对象,提取总RNA,两步法进行RT-PCR反应,用MN/CAⅨ的特异性引物扩增,2%琼脂糖凝胶电泳后观察并测序。结果 经过测序证实5例肾癌组织的RT-PCR产物中有4例可见特异的MN/CAⅨ条带,而正常肾组织的RT-PCR产物均无相应的MN/CAⅨ条带。结论 RT-PCR法检测MN/CAⅨ在肾癌组织中的表达是准确、可靠的。
Abstract:
Objective To evaluate the reliability of reverse transcription (RT) -PCR in detecting the expression of the carbonic anhydrase Ⅸ(MN/CAⅨ) gene in renal cell carcinoma (RCC).Methods MN/CAⅨwas examined in 5 cases of renal cell carcinoma tissues and 5 normal renal tissues. Primers specific for MN/CAⅨwas designed and two-step RT-PCR of the ex-tracted total RNA performed. The products was examined by 2% agrose electrophoresis and identified by sequence nanalysis.Results Specific bands of MN/CAⅨwere found in RT-PCR products of 4 case of RC C tissues, whereas could not be seen in normal renal tissues.Conclusion RT-PCR is reliable for detecting the expression of MN/CAⅨgene in RCC.

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备注/Memo

备注/Memo:
收稿日期:2003-9-27。
作者简介:姜耀东(1974- ),男,1997年毕业于第一军医大学,现为在读硕士研究生,医师,电话:020-61647097,E-mail:jyd@fimmu.com
更新日期/Last Update: 1900-01-01