[1]王万山,朴仲贤,韩明虎,等.大鼠坐骨神经再生过程中的施万细胞自噬作用[J].南方医科大学学报,2004,(01):85-87.
 WANG Wan-shan,PIAO Zhong-xian,HAN Ming-hu,et al.Autophagic effect of Schwann cells in the regeneration of rat sciatic nerves[J].Journal of Southern Medical University,2004,(01):85-87.
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大鼠坐骨神经再生过程中的施万细胞自噬作用()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2004年01期
页码:
85-87
栏目:
出版日期:
2004-01-01

文章信息/Info

Title:
Autophagic effect of Schwann cells in the regeneration of rat sciatic nerves
作者:
王万山1 朴仲贤2 韩明虎3 王启伟1 朴英杰1
1. 第一军医大学解剖教研室, 广东, 广州, 510515;
2. 汕头大学医学院中心实验室, 广东, 汕头, 515041;
3. 美国西南大学达拉斯医学中心精神病学部, 德克萨斯, 达拉斯, 75390
Author(s):
WANG Wan-shan1 PIAO Zhong-xian2 HAN Ming-hu3 WANG Qi-wei1 PIAO Ying-jie1
1. Department of Anatomy, First Military Medical University, Guangzhou 510515, China;
2. Central Laboratory, Medical College of Shantou University, Shantou 515041, China;
3. Department of Psychian, University of Texas Southwestern Medical Center, Dallas, Txeas 75390, USA
关键词:
施万细胞自噬神经再生
Keywords:
Schwann cellsautophagy nerve regeneration Wallerian
分类号:
R322.85
摘要:
目的 探讨大鼠坐骨神经再生过程中的细胞自噬作用。方法 横切大鼠坐骨神经制作wallerian变性模型,分别于造模后0、0.5、1.0、1.5、2.0、3.0、4、5、7、10、15 d取远断端组织行电镜结构观察。结果 轴突在第0.5天时从髓鞘脱离,溃变呈空泡状。第2天开始髓鞘皱褶、断裂形成碎片,神经膜细胞(Schwann cell, 施万细胞)内见大的膜结合髓鞘碎片和许多散在小碎片,并与溶酶体融合形成自噬泡,呈酸性磷酸酶(AcPase)阳性。第4天时内膜区偶见幼稚细胞,1周后见大量幼稚细胞。第7天后施万细胞内自噬泡数量开始减少。实验全程偶见巨噬细胞,内有吞噬泡。结论 大鼠坐骨神经再生过程中溃变髓鞘主要经施万细胞自噬清除,施万细胞脱分化为施万细胞祖细胞后大量增殖分化参与神经再生过程。
Abstract:
Objective To investigate the autophagic effe ct of Schwann cells in the process of rat sciatic nerve regeneration.Methods Wal lerian degeneration model was established by transecting the rat sciatic nerve. Samples from the distal stump were obtained 0, 0.5, 1, 1.5, 2, 3, 4, 5, 7, 10, and 15 days respectively after the transaction, and ultrathin sections were pre pared for examination with electron microscope.Results Axons started to separat e from the myelin sheath at day 0.5 after the transection, followed by rapid va cuolar degeneration. Since day 2, the myelin sheath folded and broke into fragme nts, and in the Schwann cells large cell membrane-bound myelin debris and many scattered small fragments could be seen to fuse with the lysosomes to form ACPa se reaction-positive autophagic vacuoles. Immature cells were occasionally seen in the endoneural space, appearing in large amount a week later. After day 7, the number of autophagic vacuoles began to diminish. In the entire course of the observation, macrophages containing autophagic vacuoles, were spotted only occa sionally.Conclusion The degenerated myelin debris are cleared mainly through t he mechanism of autophagy by the Schwann cells during regeneration of rat sciat ic nerve. Schwann cells dedifferentiate into Schwann cell precursors, which then proliferate and differentiate to take part in the regeneration of the nerves.

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备注/Memo

备注/Memo:
收稿日期:2003-10-20。
基金项目:国家重点基础发展项目(973)子课题(G1999054308-4)
作者简介:王万山(1975- ),男,第一军医大学在读博士研究生,电话:020-61647752,Email:wangwanshan@yahoo.com
更新日期/Last Update: 1900-01-01