[1]刘木彪,何援利,彭冬先.子宫内膜异位症患者腹腔液血管内皮生长因子与内抑素的表达[J].南方医科大学学报,2004,(01):69-71.
 LIU Mu-biao,HE Yuan-li,PENG Dong-xian.Expression of vascular endothelial growth factor and endostatin in peritoneal fluid of patients with endometriosis[J].,2004,(01):69-71.
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子宫内膜异位症患者腹腔液血管内皮生长因子与内抑素的表达()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2004年01期
页码:
69-71
栏目:
出版日期:
2004-01-01

文章信息/Info

Title:
Expression of vascular endothelial growth factor and endostatin in peritoneal fluid of patients with endometriosis
作者:
刘木彪 何援利 彭冬先
第一军医大学珠江医院妇产科, 广东, 广州, 510282
Author(s):
LIU Mu-biao HE Yuan-li PENG Dong-xian
Department of Obstetrics and Gynecology, Zhujiang Hospital, First Military Medica l University, Guangzhou 510282, China
关键词:
子宫内膜异位症血管内皮生长因子内抑素血管生成
Keywords:
endometriosisvascular endothelial growth factorendostatinangioge nesis
分类号:
R711.71
摘要:
目的 探讨子宫内膜异位症(内异症)患者腹腔液中血管内皮生长因子(VEGF)和内抑素(ENS)在内异症血管生成调控机制中的作用。方法 采用ELISA法检测50例内异症患者(Ⅰ~Ⅱ期26例、Ⅲ~Ⅳ期24例)和42例非内异症患者(对照组)腹腔液中VEGF和ENS的含量,分析二者浓度及其比值与内异症之间的关系。结果 内异症组腹腔液VEGFENS浓度和VEGF/ENS比值均高于对照组(P<0.01);内异症患者中Ⅲ~Ⅳ期VEGF和ENS含量均高于Ⅰ~Ⅱ期(P<0.01)而VEGF/ENS比值前者则低于后者(P<0.01)。增殖期与分泌期比较,除内异症组VEGF水平前者高于后者外(P<0.05)对照组VEGF和两组ENS水平两期比较均无差异(P>0.05)。结论 VEGF和ENS同时参与了内异症的血管生成调控而两者表达水平的失衡可能才是内异症发病的关键。
Abstract:
Objective To investigate the r el ationship of vascular endothelial growth factor (VEGF) and endostatin with en-do metriosis.Methods The levels of vascular endothelial growth factor and endostat in were measured using ELISA in 50 women with endometriosis (stage Ⅰ~Ⅱ26 case s and stage Ⅲ~Ⅳ24 cases) in comparison with 42 women without en-dometriosis.Results The VEGF, endostatin concentrations and VEGF/endostatin ratio in endometr iosis group were signifi-cantly higher than the mean value in the control group (P<0.01). The VEGF and endostatin levels in stages Ⅲand Ⅳwere both significan tly higher than those in stagesⅠandⅡ(397±81 pg/ml vs315±64 pg/ml, 125±62 ng /ml vs66±40 ng/ml, P<0.01). However, the VEGF/endostatin ratio was lower in th e advanced stages. There was no significant difference between follicular phase and luteal phase except that the VEGF level in follicular phase was higher than that in luteal phases.Conclusions Both VEGF and endostatin may play a role in the regulation of angiogenesis of endometriosis, and the balance of angiogenic stimu-lators and inhibitors may be critical to the development of endometriosis.

参考文献/References:

[1] Pepper MS. Manipulating angiogenesis. From basic science to the bedside[J]. Arterioscler Thromb Vasc Biol,1997,17(5):605-19.
[2] American society for Reproduction Medicine. Revised American society for reproduction medicine classification of endometriosis[J].Fetil Steril,1997,67(8):817-21.
[3] Dvorak HF,Brown LF,Detmar M,et al. Vascular permeability factor/vascular endothelial growth factor,microvascular hyperpermeability,and angiogenasis[J]. Am J Pathol,1995,146(11):1029-39.
[4] Dhanabal M,Ramchandran R,Waterman MJ,et al. Endostatin induces endothelial cell apoptosis[J]. J Biol Chem,1999,274(17):11721-6.
[5] 夏虎,张琳,文金序,等.人内皮抑素在毕赤酵母中的表达、纯化及其对小鼠肺腺癌LA795细胞生长的抑制[J].第一军医大学学报,2002,22(5):393-6.Xia H,Zhang L,Wen JX,et al. Expression and purification of human endostatin in Pichia pastoris and its inhibition on the growth of mouse pulmonary adenocarcinoma cell line LA795[J]. J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao,2002,22(5):393-6.
[6] Mclaren J. Vascular endothelial growth factor and endometriotic angiogenesis[J]. Hum Reprod Update,2000,6(1):44-5.
[7] Giudice LC,Tazuke SI,Swiersz LT. Status of current research on endometriosis[J]. J Reprod Med,1998,43(3 Suppl):252-62.
[8] 宗利丽,李亚里,哈小琴.子宫内膜异位症患者腹水与血清中肝细胞生长因子的检测[J].第一军医大学学报,2003,23(8):757-60.Zong LL,Li YL,Ha XQ. Determination of HGF concentration in serum and peritoneal fluid in women with endometriosis[J]. J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao,2003,23(8):757-60.
[9] Kupker W,Schultze-Mosgau A,Diedrich K. Paracrine changes in the peritoneal environment of women with endometriosis[J]. Hum Reprod Update,1998,4(5):719-23.
[10] Barcz E,Kaminski P,Marianowski L. VEGF concentration in peritoneal fluid of patients with endometriosis[J]. Ginekol Pol,2001,72(5):442-8.
[11] 谭先杰,郎景和,刘东远,等.血管内皮生长因子及血小板反应素在异位内膜组织中的表达[J].中华妇产科杂志,2001,36(12):737-40.Tan XJ,Lang JH,Lin DY,et al. Expression of vascular endothelial growth factor and thrombospondin-1 mRNA in patients with endometriosis[J]. Chin Obst Gynecol J,2001,36(12):737-40.
[12] Shaarawy M,E1-Sharkawy SA. Biomarkers of intrinsic angiogenic and anti-angiogenic activity in patients with endometrial hyperplasia and endometrial cancer[J]. Acta Oncol,2001,40(4):513-8.
[13] 徐丹,孟凡义,张燕.血管内皮生长因子在急性髓系白血病细胞中的表达[J].第一军医大学学报,2002,22(4):357-9.Xu D,Meng FY,Zhang y. Expression of vascular endothelial growth factor in bone marrow cells of patients with acute myeloid leukemia[J]. J First Mil Med Univ/Di Yi.Jun Yi Da Xue Xue Bao,2002,22(4):357-9.

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备注/Memo

备注/Memo:
收稿日期:2003-8-21。
基金项目:广东省科技厅科技计划项目(2003B30502)
作者简介:刘木彪(1972- ),男,1995年毕业于第一军医大学,现为在读硕士研究生,主治医师、讲师,主要研究方向:妇科内镜技术及子宫内膜异位症,电话:020-61643360
更新日期/Last Update: 1900-01-01