[1]曾玉梅,王思思,封慕茵,等.SETD2基因敲除前后鼻咽癌细胞中定量蛋白组学研究及差异信号富集[J].南方医科大学学报,2019,(10):1191.[doi:10.12122/j.issn.1673-4254.2019.10.10]
点击复制

SETD2基因敲除前后鼻咽癌细胞中定量蛋白组学研究及差异信号富集()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2019年10期
页码:
1191
栏目:
出版日期:
2019-10-15

文章信息/Info

Title:
Quantitative proteomics and differential signal enrichment in nasopharyngeal carcinoma cells with or without SETD2 gene knockout
作者:
曾玉梅王思思封慕茵邵钟铭袁建玲申志华揭伟
关键词:
鼻咽癌SETD2TMT标记定量蛋白组学生物信息学
Keywords:
nasopharyngeal carcinoma SETD2 TMT-tagged quantitative proteomics bioinformatics
DOI:
10.12122/j.issn.1673-4254.2019.10.10
摘要:
目的分析甲基转移酶SETD2 表达改变对人鼻咽癌(NPC)细胞中蛋白表达谱的影响并富集差异信号通路。方法提取 SETD2基因敲除细胞株CNE1SETD2-KO和野生型细胞CNE1WT的总蛋白,利用Tandem Mass Tag(TMT)标记蛋白质定量技术和串联 质谱分析技术筛选出差异表达蛋白质,应用GO数据库对差异表达蛋白质进行注释和富集,应用KEGG数据库进行差异蛋白质 涉及信号通路富集。结果以表达倍数(FC)≥1.2倍且P<0.05为筛选标准,SETD2基因敲除的CNE1细胞鉴别出2049个差异表 达蛋白质,其中904个表达上调,1145个表达下调。GO功能注释结果表明,SETD2敲除后在生物过程(细胞过程及调节,细胞运 动、代谢过程及细胞组分的生物合成)、分子功能(催化活性及分子结合、转录因子活性)和细胞组分(胞膜、细胞器、大分子复合 物)等具有特征性。KEGG信号富集结果表明SETD2 敲除主要影响与肿瘤关系密切的信号有MAPK、PI3K-Akt、Ras、Rap1、 mTOR、Hippo、HIF-1、Wnt、AMPK、FoxO、ErbB、p53、JAK-STAT等。结论SETD2敲除后显著改变了NPC细胞中蛋白质表达特 征并影响多条与肿瘤关系密切的信号通路,研究结果为NPC的发病机制和治疗标靶筛选提供了参考。
Abstract:
Objective To analyze the effects of alterations in the expressions of methyltransferase SETD2 on protein expression profiles in human nasopharyngeal carcinoma (NPC) cells and enrich the differential signaling pathways. Methods The total protein was extracted from SETD2-knockout cell line CNE1SETD2-KO and the wild-type cell line CNE1WT, and the differentially expressed proteins were screened by tandem mass tag (TMT) labeled protein quantification technique and tandem mass spectrometry. GO analysis was used to annotate and enrich the differentially expressed proteins, and the KEGG database was used to enrich and analyze the pathways of the differential proteins. Results With a fold change (FC)≥1.2 and P<0.05 as the screening standard, 2049 differentially expressed proteins were identified in CNE1SETD2-KO cells, among which 904 were up-regulated and 1145 were down-regulated. GO functional annotation results indicated that SETD2 knockout caused characteristic changes in multiple biological processes (cell processes and regulation, cell movement, metabolic processes, and biosynthesis of cellular components), molecular functions (catalytic activity and molecular binding, transcription factor activity), and cellular components (cell membrane, organelle, macromolecular complex). KEGG analysis showed that the differentially expressed proteins were involved in an array of signaling pathways closely related to tumors, including MAPK, PI3K-Akt, Ras, Rap1, mTOR, Hippo, HIF-1, Wnt, AMPK, FoxO, ErbB, P53 and JAK-STAT. Conclusion SETD2 knockout significantly changes the protein expression characteristics of NPC cells and affects a number of signal pathways closely related to tumors. The results provide evidence for investigation of the pathogenesis and therapeutic target screening of NPC.

相似文献/References:

[1]陈智贤,孙爱民,陈勇,等.放射敏感性、X线辐射剂量对鼻咽癌细胞miR-7表达的影响[J].南方医科大学学报,2010,(08):1810.
 CHEN Zhi-xian,SUN Ai-min,CHEN Yong,et al.Effects of radiosensitivity and X-ray dose on miR-7 expression in nasopharyngeal carcinoma[J].Journal of Southern Medical University,2010,(10):1810.
[2]熊艺颖,黄仲曦.来自不同地区的两组鼻咽癌活检组织样本共同失调的基因功能及通路[J].南方医科大学学报,2011,(03):414.
[3]刘晓东,季明芳.p53通路相关基因和EZH2在鼻咽癌中的表达关系及意义[J].南方医科大学学报,2011,(06):1029.
[4]江庆萍,刘少颜,何秀芳,等.鼻咽癌中MAP3K5和Epstein-Barr病毒编码的miR-BART22表达的关系及意义[J].南方医科大学学报,2011,(07):1146.
[5]柏志全,李华荣,张海峰,等.氯通道在藤黄酸诱导低分化鼻咽癌细胞凋亡中的作用[J].南方医科大学学报,2011,(08):1304.
[6]陈静平,龙军,张宏征.EB病毒壳抗原抗体定性检测在鼻咽癌筛查中的作用[J].南方医科大学学报,2011,(09):1637.
[7]王宏梅,石玉生,李启生,等.毛细管扩张症突变基因启动子区单核苷酸多态性位点rs189037与广东鼻咽癌相关性研究[J].南方医科大学学报,2011,(11):1863.
[8]李贵平,黄凯,张辉,等.188Re-Herceptin放免导向治疗鼻咽癌裸鼠模型的实验研究[J].南方医科大学学报,2006,(04):459.
 LI Gui-ping,HUANG Kai,ZHANG Hui Departmant of Nuclear Medicine,et al.Efficacy of 188Re-Herceptin radioimmunotherapy in nude mouse model of nasopharyngeal carcinoma[J].Journal of Southern Medical University,2006,(10):459.
[9]韩春,王爽,刘莉,等.鼻咽癌细胞肝转移亚株的建立[J].南方医科大学学报,2006,(05):654.
 HAN Chun,WANG Shuang,LIU Li,et al.Establishment of hepatic metastatic subline of nasopharyngeal carcinoma cell line[J].Journal of Southern Medical University,2006,(10):654.
[10]王剑,吴德华,陈龙华.铜绿假单胞菌制剂抑制鼻咽癌细胞体外增殖[J].南方医科大学学报,2012,(04):544.

更新日期/Last Update: 1900-01-01