[1]王剑,曹传辉,曾钦,等.长链非编码RNA-UFC1通过GSK-3β/β-catenin信号轴促进肝癌细胞的侵袭和转移[J].南方医科大学学报,2019,(06):679.[doi:10.12122/j.issn.1673-4254.2019.06.08]
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长链非编码RNA-UFC1通过GSK-3β/β-catenin信号轴促进肝癌细胞的侵袭和转移()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2019年06期
页码:
679
栏目:
出版日期:
2019-06-16

文章信息/Info

Title:
Long noncoding RNA UFC1 promotes metastasis and invasion of hepatocellular carcinoma cells in vitro via GSK-3β/β-catenin axis
作者:
王剑曹传辉曾钦董忠谊
关键词:
lincRNA-UFC1β-catenin磷酸化GSK3β肝细胞癌细胞侵袭转移
Keywords:
lincRNA-UFC1 β-catenin p-GSK3 hepatocellular carcinoma tumor progression
DOI:
10.12122/j.issn.1673-4254.2019.06.08
摘要:
目的探讨长链非编码RNA-UFC1在肝癌侵袭和转移中的作用及机制。方法应用lincRNA-UFC1慢病毒载体转染人肝 癌细胞株Huh7 构建lincRNA-UFC1 过表达组和对照组;应用lincRNA-UFC1 干扰载体转染人肝癌细胞株BEL-7402 构建 lincRNA-UFC1 干扰组和干扰对照组(scramble),通过实时定量PCR实验分析两种肝癌细胞中lincRNA-UFC1 的表达水平。 Transwell 及划痕实验检测lincRNA-UFC1 过表达组及干扰组相比对照组侵袭迁移能力的变化。Western blot 检测lincRNAUFC1 过表达组及干扰组相比对照组中GSK-3β/β-catenin蛋白的表达变化。利用GSK-3β/β-Catenin信号通路抑制剂XAV939阻 断后,观察Transwell 及划痕实验中lincRNA-UFC1 过表达对肝癌细胞侵袭迁移能力的影响。结果在肝细胞癌中,过表达 lincRNA-UFC1 相较于对照组,肝癌细胞的侵袭转移能力明显增加(P<0.001),而沉默lincRNA-UFC1 的表达相较于scramble 组,肝癌细胞侵袭转移能力明显减弱(P<0.001)。Western blot结果显示,过表达lincRNA-UFC1与对照组相比较,侵袭转移相关 蛋白β-catenin和p-GSK-3β表达明显上调(P<0.001),而lincRNA-UFC1沉默表达后,结果相反。利用GSK-3β/β-Catenin信号通 路抑制剂XAV939 处理肝癌细胞可以逆转lincRNA-UFC1 促进肝癌细胞侵袭转移的功能(P<0.001)。结论在肝细胞癌中 lincRNA-UFC1通过上调磷酸化GSK-3β及β-catenin促进肝癌细胞侵袭转移。
Abstract:
Objective To explore the role of Long noncoding RNA UFC1 (lincRNA-UFC1) in modulating the metastasis and invasion of hepatocellular carcinoma (HCC) cells and the underlying mechanism. Methods Human HCC cell line Huh7 was infected with the lentiviral vector carrying lincRNA-UFC1 to obtain a cell line with lincRNA-UFC1 overexpression. A short hairpin RNA (shRNA) targeting lincRNA-UFC1 was delivered in human HCC BEL-7402 cells via a lentiviral vector to obtain a cell line with lincRNA-UFC1 knockdown. Expression levels of lincRNA-UFC1 in the two HCC cell lines were detected using real-time PCR, and the changes in the cell invasion and migration in response to lincRNA-UFC1 overexpression or knockdown were analyzed using Transwell and wound-healing assays. The expressions of GSK-3β/β-catenin-related proteins in the cells were detected with Western blotting. XAV-939, a GSK-3β/β-catenin inhibitor, was used for assessing the impact of lincRNAUFC1 overexpression on the invasion and migration of the HCC cells through Transwell and wound-healing assays. Results Overexpression of lincRNA-UFC1 significantly promoted the invasion and migration of Huh7 cells as compared with the control cells (P<0.001), while lincRNA-UFC1 knockdown obviously suppressed the invasion and migration of BEL-7402 cells (P<0.001). The results of Western blotting showed that the expressions of proteins associated with the cell invasion and migration, namely β-catenin and P-GSK-3β, were significantly upregulated in response to lincRNA-UFC1 overexpression, and were obviously lowered after lincRNA-UFC1 knockdown. Treatment of the cells with XAV-939 significantly reversed the effect of lincRNA-UFC1 overexpression on the cell invasion and migration (P<0.001). Conclusion lincRNA-UFC1 overexpresison promotes cell invasion and migration through the GSK-3β/β-catenin axis in HCC cells in vitro.

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更新日期/Last Update: 1900-01-01