[1]李利,张婷婷,陈玉华,等.转录因子SOX12在肺腺癌中的表达及其与临床预后的关系[J].南方医科大学学报,2019,(02):186.[doi:10.12122/j.issn.1673-4254.2019.02.10]
点击复制

转录因子SOX12在肺腺癌中的表达及其与临床预后的关系()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2019年02期
页码:
186
栏目:
出版日期:
2019-02-28

文章信息/Info

Title:
Expression of transcription factor SOX12 in lung adenocarcinoma and its clinical significance
作者:
李利张婷婷陈玉华宋佳孟瑶刘姝谢剑明
关键词:
SOX12肺腺癌预后
Keywords:
SOX12 lung adenocarcinoma prognosis
DOI:
10.12122/j.issn.1673-4254.2019.02.10
摘要:
目的探讨转录因子SOX12在肺腺癌中的表达及其与临床预后的关系。方法采用大型癌症基因组数据库分析SOX12在 肺腺癌中的表达水平,然后分别采用免疫组织化学(IHC)和半定量PCR方法检测36例肺腺癌癌组织、15例距肿瘤边缘5 cm以 上癌旁正常组织和21例正常肺组织中SOX12 的表达情况,同时通过Kaplan-Meier Plotter 数据库获取了866例肺腺癌患者和 675例肺鳞癌患者的生存数据,分析SOX12基因表达水平对患者总生存期(OS)和进展后生存期(PPS)的影响。结果TCGA数 据库和GEO(GSE40419)样本集提示SOX12的表达水平在肺腺癌组中显著高于癌旁组(P<0.001),同时IHC和半定量PCR结果 均显示SOX12 在肺腺癌组织中的表达高于癌旁正常组织和正常肺组织,差异具有统计学意义(P<0.001);分析Kaplan-Meier Plotter数据库显示SOX12基因表达水平高的肺腺癌患者较表达水平低的患者OS和PPS短,差异具有统计学意义(P<0.05);而 SOX12基因表达水平对肺鳞癌患者的OS和PPS均无明显影响。结论转录因子SOX12在肺腺癌组织中呈明显高表达水平,且 其表达水平与患者的生存和预后关系密切,SOX12可能成为预测肺腺癌发生发展的辅助指标。
Abstract:
Objective To explore the expression patterns of transcription factor SOX12 in lung adenocarcinoma and its significance in the diagnosis and prognosis of the malignancy. Methods Large cancer genome databases were used to analyze SOX12 expression level in lung adenocarcinoma. Immunohistochemistry (IHC) and semiquantitative PCR were used to detect the expression of SOX12 in 36 specimens of lung adenocarcinoma tissues, 15 adjacent tissues and 21 normal lung tissues. The prognostic value of SOX12 in lung adenocarcinoma and lung squamous cell carcinoma were analyzed using Kaplan-Meier Plotter database, and the relationship between SOX12 expression and the overall survival (OS) and progression free survival (PPS) of the patients were analyzed. Results Analysis of TCGA database and GEO (GSE40419) database showed that SOX12 expression levels were significantly higher in in lung adenocarcinoma than in normal lung tissues (P<0.001). The results of IHC and semiquantitative PCR revealed that SOX12 was expressed at significantly higher levels in lung adenocarcinoma than in normal lung tissues (P<0.001). Kaplan-Meier survival analysis showed that patients with lung adenocarcinoma positive for SOX12 had a significantly shorter OS and PPS time than those negative for SOX12 (P<0.05), but SOX12 positivity did not significantly affect OS and PPS of patients with lung squamous cell carcinoma. Conclusion High expression levels of SOX12 in lung adenocarcinoma are significantly associated with a poor OS of the patients, suggesting the value of SOX12 to assist in early diagnosis and prognostic evaluation of lung adenocarcinoma.

相似文献/References:

[1]赵晓,燕翔,孙胜杰,等.晚期肺腺癌患者外周血T淋巴细胞亚群检测的临床意义[J].南方医科大学学报,2012,(05):752.
[2]宋海星,胡洪华.羟基喜树碱抑制肺癌A549细胞的体外增殖并下调Bcl-2基因的表达[J].南方医科大学学报,2012,(09):1341.
[3]李慧灵,邓秋华,黄丽燕,等.磷脂酰肌醇蛋白聚糖-3在肺鳞癌和腺癌中的不同表达及其与预后的关系[J].南方医科大学学报,2013,(02):212.
[4]傅伟,彭辉,陈志强,等.罕见肺腺癌前列腺转移1例报告并文献复习[J].南方医科大学学报,2014,(02):289.
[5]徐小艳,刘红伟,姜黄,等.联合检测细胞蜡块中的napsin A和甲状腺转录因子-1有助于肺腺癌胸水的诊断[J].南方医科大学学报,2015,(11):1610.
[6]潘莹,黄思超,王霞,等.NF-κB亚单位p50/p65激活促进肺腺癌H1650细胞吉非替尼耐药[J].南方医科大学学报,2018,(05):584.
[7]王玉环,张淑华,穆淑坤,等.去泛素化酶USP33通过下调SLIT2/ROBO1信号通路抑制肺腺癌的侵袭和转移[J].南方医科大学学报,2018,(08):956.
[8]何萍,顾霞,曾欣,等.肺原位腺癌、微浸润腺癌及浸润性腺癌中淋巴管密度的变化及其调控因子[J].南方医科大学学报,2018,(11):1349.[doi:10.12122/j.issn.1673-4254.2018.11.12]
[9]彭淑贤,李浔,刘琴,等.转录组数据分析肺腺癌和肺鳞状细胞癌中基因的差异表达[J].南方医科大学学报,2019,(06):641.[doi:10.12122/j.issn.1673-4254.2019.06.03]

更新日期/Last Update: 1900-01-01