[1]叶桂荣,周敏捷,于立新,等.肾移植受者、慢性肾脏病患者和健康对照者肠道菌群的对比[J].南方医科大学学报,2018,(12):1401.[doi: 10.12122/j.issn.1673-4254.2018.12.01]
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肾移植受者、慢性肾脏病患者和健康对照者肠道菌群的对比()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2018年12期
页码:
1401
栏目:
出版日期:
2018-12-31

文章信息/Info

Title:
Gut microbiota in renal transplant recipients, patients with chronic kidney disease and healthy subjects
作者:
叶桂荣周敏捷于立新叶俊生姚琳史丽莎
关键词:
肠道菌群肾移植慢性肾脏病16S rRNA测序
Keywords:
gut microbiota renal transplantation chronic kidney disease 16S rRNA sequencing
DOI:
10.12122/j.issn.1673-4254.2018.12.01
摘要:
目的研究表明肠道菌群与移植受体的免疫调节相关,但目前对肾移植受者肠道菌群的组成及功能的研究却很少。方法 我们通过对粪便标本的16S rRNA V3可变区进行深度测序,分析16例肾移植受者(TR)肠道菌群的组成和功能,并与84例慢性 肾脏病(CKD)患者和53例健康对照人群肠道菌群进行比较,以进一步了解肠道菌群的特点。结果TR与CKD肠道菌群结构 相似,菟丝子科、漆树科、芸香科和粪杆菌的丰度显著降低。与对照组相比,TR、CKD中类杆菌、蛋白杆菌、梭形杆菌和肠杆菌科 的丰度均有所增加。功能比较显示TR和CKD患者糖代谢显著增加,辅因子、维生素、细胞活力和遗传信息处理显著降低。TR 和CKD相比也有轻微差异。TR中蛋白细菌和肠杆菌科的丰度,以及涉及转运系统成员和碳水化合物代谢的途径都显著高于 TR。我们发现拉氏囊藻科和微囊藻科的几个有益菌属与临床标志物(血清肌酐和血尿素氮)呈负相关。结论本研究对TR中 肠道菌群的组成及功能进行了分析,提示TR中肠道菌群的改变与患者临床表现密切相关。对肾移植与肠道菌群之间的相关性 进行更前瞻性的研究将有助于预测移植术后的临床反应。
Abstract:
Objective Recent studies have shown that gut microbiota is associated with immunomodulation in transplant recipients, but the composition and function of gut microbiota in renal transplant recipients have not been understood. Methods We analyzed the composition and function of gut microbiota in the fecal samples from 16 renal transplant (RT) recipients by deep sequencing of the 16S rRNA V3 variable region. The gut microbiota of RT recipients was compared to that of 84 patients with chronic kidney disease (CKD) and 53 healthy subjects. Results The overall microbial structure of RT recipients was similar to that of CKD. The abundance of Firmicutes, Lachnospiraceae, Ruminococcaceae and Faecalibacterium was decreased and that of Bacteroidetes, Proteobacteria, Clostridiales, and Enterobacteriaceae was increased significantly in RT recipients and CKD patients compared with the healthy control subjects. Functional comparison revealed significantly enhanced carbohydrate metabolism and decreased metabolism of cofactors, vitamins, cell motility and genetic information processing in RT recipients and CKD patients. RT recipients and CKD patients also showed slight differences in that the abundance of Proteobacteria and Enterobacteriaceae and the pathways involving transport system members and carbohydrate metabolism were much greater in the former. We found that several beneficial genera in the Lachnospiraceae and Veillonellaceae were negatively correlated with such clinical markers as serum creatinine and blood urea nitrogen. Conclusion Our results suggested that alterations in the composition and function of gut microbiota are significantly correlated with the clinical conditions of in RT recipients, and future prospective studies of these correlations may provide evidence for predicting the clinical outcomes of RT recipients.

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更新日期/Last Update: 1900-01-01