[1]陈占军,相乐阳,黄毓,等.长链非编码RNA linc00261在肝细胞癌中的表达及意义[J].南方医科大学学报,2018,(10):1179.[doi:10.12122/j.issn.1673-4254.2018.10.05]
点击复制

长链非编码RNA linc00261在肝细胞癌中的表达及意义()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2018年10期
页码:
1179
栏目:
出版日期:
2018-10-16

文章信息/Info

Title:
Expression of long noncoding RNA linc00261 in hepatocellular carcinoma and its association with postoperative outcomes
作者:
陈占军相乐阳黄毓方英豪李湘竑杨定华
关键词:
linc00261肝细胞癌增殖迁移侵袭
Keywords:
linc00261 hepatocellular carcinoma proliferation migration invasion
DOI:
10.12122/j.issn.1673-4254.2018.10.05
摘要:
目的研究长链非编码RNA linc00261 在肝细胞癌(HCC)中的表达、功能及其临床意义。方法采用实时荧光定量PCR (qRT-PCR)法检测linc00261在74例肝癌、癌旁肝组织中的表达;采用卡方检验分析其表达量与临床病理指标的相关性;应用 COX回归模型分析其表达水平与肝癌患者术后预后的关系;qRT-PCR检测linc00261在5株肝癌细胞株中的表达;在肝癌细胞 株MHCC-LM3和SNU-449中采用siRNA(si-linc00261-1,si-linc00261-2,si-NC和空白对照组)和Lipofectamine3000进行转染 敲低linc00261,活细胞计数(CCK8)、Transwell实验分别研究linc00261对肝癌细胞增殖,侵袭迁移能力的影响。结果HCC组 织中linc00261的表达水平与肝癌患者术前血清AFP(P=0.032)、肿瘤大小(P=0.007)、有无微血管癌栓(P=0.01)、TNM分期(P= 0.02)显著相关;HCC组织中linc00261的低表达与患者的不良预后相关,其术后无瘤生存期明显缩短(P<0.05),是影响HCC患 者术后无瘤生存期的一个独立危险因素;下调linc00261的表达可促进肝癌细胞株MHCC-LM3(P<0.01)和SNU-449(P<0.001) 的迁移和侵袭能力,对细胞增殖无明显影响(P>0.05)。结论Linc00261有望成为肝癌切除术后的一种新的预后指标。
Abstract:
Objective To investigate the expression of long noncoding RNA linc00261 in hepatocellular carcinoma (HCC) and its correlation with the clinicopathological features and postoperative outcomes of the patients. Methods Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the expression of linc00261 in surgical specimens of HCC and adjacent tissues from 74 patients. The correlation of the expression level of linc00261 in HCC with the clinicopathological parameters of the patients was analyzed using Chi-square test. The Cox’s proportional hazards regression model was used to assess the value of linc00261 in predicting the prognosis of HCC patients after operation. The expression of linc00261 was also examined in 5 HCC cell lines using qRT-PCR. The HCC cell lines MHCC-LM3 and SNU-449 were transfected with small interfering RNAs targeting linc00261 for linc00261 knockdown, and the changes in the cell proliferation, migration and invasion abilities were observed using CCK-8 assay and Transwell assay. Results The expression level of linc00261 in HCC was significantly correlated with AFP (P=0.032), tumor size (P=0.007), microscopic vascular invasion (MVI; P=0.01), and TNM stage (P=002). The patients with lowered expressions of linc00261 in HCC tissues had a significantly shortened tumor-free survival time (P<0.05), and a lowered expression of linc00261 was identified as an independent risk factor affecting postoperative recurrence-free survival time of the patients (P<0.05). In HCC cell lines MHCC-LM3 and SNU-449 cells, linc00261 knockdown obviously promoted the cell migration and invasion (P<0.01) but did not significantly affect cell proliferation (P>0.05). Conclusion Linc00261 may serve as a new prognostic biomarker for predicting the postoperative outcomes of the patients with HCC.

相似文献/References:

[1]李湘竑,钟克波,刘延,等.索拉菲尼治疗肝癌肝移植术后肿瘤复发患者的疗效及安全性分析[J].南方医科大学学报,2011,(09):1608.
[2]郭丹,陈娜娜,侯连兵,等.前列腺素E2活化EP2受体促进肝癌细胞增殖[J].南方医科大学学报,2011,(09):1564.
[3]陈可和,梁博,邹镇洪,等.Rheb和Rheb’D60K突变基因重组慢病毒载体的构建、鉴定及其对肝癌细胞凋亡的影响[J].南方医科大学学报,2012,(03):341.
[4]赵爱国,吴曙光.沉默CDK7基因对HepG2细胞株的抗增殖作用[J].南方医科大学学报,2005,(01):58.
 ZHAO Ai-guo,WU Shu-guang.Effect of CDK7 gene silencing against hepatoblastoma HepG2 cell proliferation[J].Journal of Southern Medical University,2005,(10):58.
[5]金吴东,陈龙华,穆峰.外源性mTκα基因抑制肝癌细胞HepG2的生长[J].南方医科大学学报,2004,(10):1133.
 JIN Wu-dong,CHEN Long-hua,MU Feng.Exogenous mTκα gene inhibits the growth of HepG2 heptocellular carcinoma cells[J].Journal of Southern Medical University,2004,(10):1133.
[6]余元龙,季明芳,何洁冰,等.紫杉醇、5-氟脲嘧啶抑制肝癌细胞BEL-7402生长和诱导凋亡的比较[J].南方医科大学学报,2005,(07):864.
 YU Yuan-long,JI Ming-fang,HE Jie-bing,et al.Effects of paclitaxel and 5-fluorouracil on growth inhibition and apoptosis of hepatoma cells:a comparative study[J].Journal of Southern Medical University,2005,(10):864.
[7]陈志丰,梁宏,张祥松,等.肝癌治疗后甲胎蛋白增高患者的18F-FDG PET/CT与增强CT对照研究[J].南方医科大学学报,2012,(11):1615.
[8]李超,涂康生,郑鑫,等.MicroRNA-218在肝细胞癌中的表达及功能[J].南方医科大学学报,2013,(08):1127.
[9]黄俊海,周杰.符合Milan标准肝细胞癌患者行肝移植和肝切除术预后分析[J].南方医科大学学报,2014,(03):406.
[10]谢亮海,蒋晗,吴福生.Wnt/β-catenin信号通路在肝细胞癌中的促进作用[J].南方医科大学学报,2014,(06):913.

更新日期/Last Update: 1900-01-01