[1]黄丽,王春,李言,等.针刺预处理对脑缺血大鼠小脑浦肯野细胞动作电位的影响[J].南方医科大学学报,2018,(06):677.
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针刺预处理对脑缺血大鼠小脑浦肯野细胞动作电位的影响()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2018年06期
页码:
677
栏目:
出版日期:
2018-06-30

文章信息/Info

Title:
Effect of acupuncture pretreatment on action potential of cerebellar Purkenje cells in ex vivo ischemic rat brain slices
作者:
黄丽王春李言赵士弟
关键词:
脑缺血针刺预处理浦肯野细胞百会穴足三里穴膜片钳动作电位
Keywords:
ischemia acupuncture pretreatment cerebellar Purkenje cells Baihui acupoint Zusanli acupoint patch-clamp action potential
摘要:
目的探讨针刺预处理特定穴位对脑缺血早期大鼠小脑浦肯野细胞动作电位发放的影响。方法40只雄性SD大鼠随机分 为单纯脑缺血组(包括正常对照组和脑缺血组)和针刺预处理脑缺血组(包括针刺预处理脑缺血前和脑缺血后组),每组20只。 采用减慢脑片灌流速度模拟在体缺血性脑中风的脑缺血模型,针刺预处理组针刺部位选择百会穴和双侧足三里穴,每日2次,间 歇5 min捻转1次,留针30 min,连续针刺7 d。应用全细胞膜片钳记录方法,观察各组大鼠小脑浦肯野细胞缺血早期动作电位发 放的变化。结果与正常对照组比较,单纯脑缺血早期浦肯野细胞动作电位发放表现为峰间距缩短、编码精确性降低和阈电位 降低(P<0.01),即浦肯野细胞过度兴奋,而针刺预处理能够减轻脑缺血早期浦肯野细胞动作电位峰间距缩短、编码精确性降低 和阈电位降低的程度。结论针刺预处理特定穴位能够改善小脑浦肯野细胞的兴奋性损伤,稳定脑缺血后浦肯野细胞动作电位 的发放,对缺血性脑中风起到一定的保护作用。
Abstract:
Objective To investigate the effect of acupuncture pretreatment at specific acupoints on action potential of cerebellar Purkenje cells in rats early after cerebral ischemia. Methods Forty male SD rats were randomized into control group, ischemia group, acupuncture pretreatment group and acupuncture pretreatment plus ischemia group. The rats in acupuncture groups received acupuncture pretreatment at Baihui and bilateral Zusanli twice daily for 7 consecutive days, after which brain slices were prepared and perfused at a lowered rate to simulate in vivo ischemic stroke. Microelectrode and whole cell current clamp technique were used for recording the action potentials of cerebellar Purkenje cells to detect changes in spike encoding of the cells. Results Compared with those in the control group, the rat brain slices early after simulated ischemia showed significantly shortened inter-spike intervals, increased standard deviation of spike timing and decreased voltage of threshold potentials (P<0.01), suggesting overexcitation of the Purkinje cells. Acupuncture pretreatment at Baihui and bilateral Zusanli obviously suppressed overexcitation of the Purkinje cells in response to ischemia. Conclusion Acupuncture pretreatment at Baihui and bilateral Zusanli can improve ischemic stroke by suppressing overexcitation of Purkenje cells in rats.

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更新日期/Last Update: 1900-01-01