[1]刘亚宸,陈渠,杨兴龙,等.利用CRISPR/Cas9技术建立NOD/SCID/IL2Rγ-/-免疫缺陷小鼠[J].南方医科大学学报,2018,(06):639.
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利用CRISPR/Cas9技术建立NOD/SCID/IL2Rγ-/-免疫缺陷小鼠()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2018年06期
页码:
639
栏目:
出版日期:
2018-06-30

文章信息/Info

Title:
Generation of a new strain of NOD/SCID/IL2Rγ-/- mice with targeted disruption of Prkdc and IL2Rγ genes using CRISPR/ Cas9 system
作者:
刘亚宸陈渠杨兴龙汤清双姚开泰徐洋
关键词:
基因工程CRISPR/Cas9系统免疫缺陷小鼠cNSG品系移植
Keywords:
gene editing CRISPR/Cas9 system immunodeficiency cNSG strain xenotransplantation
摘要:
目的NOD/SCID/IL2Rγ-(/- NSG)小鼠品系是最严重的免疫缺陷品系,广泛应用于异种移植的研究。然而,SCID突变是 Prkdc 基因的一个自发性突变,会导致T细胞发育阻滞后渗漏而且很难进行基因型鉴定。因此,开发一个敲除Prkdc和IL2Rγ基 因的新品系NSG小鼠非常重要。方法使用CRISPR/Cas9系统实现Prkdc和IL2Rγ基因的靶向敲除。将基因敲除小鼠与NOD 背景小鼠杂交,我们产生了新品系NOD/SCID/IL2Rγ-(/- NSG)小鼠,称为cNSG(中国NSG)小鼠。结果cNSG 小鼠完全缺乏T 细胞,B细胞以及NK细胞而且表达NOD突变。体内成瘤实验证实cNSG鼠是一种比免疫缺陷裸鼠更有效的异种移植模型。 结论cNSG 小鼠与Jackson实验室的NSG鼠的免疫缺陷一致,有望成为中国生物医学研究的重要异种移植模型。
Abstract:
Objective The NOD/SCID/IL2Rγ-/- (NSG) mouse strain is the most widely used immunodeficient strain for xenograft transplantation. However, the existing SCID mutation is a spontaneous mutation of the Prkdc gene, which leads to leaky T cell developmental block and difficulty in genotyping. It is therefore important to develop a new strain of NSG mice with targeted disruption of Prkdc and IL2Rγ genes. Methods Targeted disruption of Prkdc and IL2Rγ genes was achieved using the CRISPR/ Cas9 system. By intercrossing the knockout and NOD mice, we obtained a novel strain of NOD/SCID/IL2Rγ-/- (NSG) mice, denoted as cNSG (Chinese NSG) mice. Results In addition to the NOD mutation, cNSG mice exhibited a complete absence of T cells, B cells and NK cells. cNSG mice allowed more efficient engraftment of human cancer cells than the commonly used immunodeficient nude mice. Conclusion cNSG mice will provide an important xenotransplantation model for biomedical research.

相似文献/References:

[1]王成博,康巧珍,丁聪,等.利用CRISPR/Cas9系统构建稳定敲除4.1R基因的RAW264.7细胞株[J].南方医科大学学报,2017,(12):1609.

更新日期/Last Update: 1900-01-01