[1]万勇坤,李徽徽,左琳,等.日本血吸虫半胱氨酸蛋白酶抑制剂干预脂多糖诱导的小鼠脓毒症[J].南方医科大学学报,2018,(05):625.
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日本血吸虫半胱氨酸蛋白酶抑制剂干预脂多糖诱导的小鼠脓毒症()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2018年05期
页码:
625
栏目:
出版日期:
2018-05-15

文章信息/Info

Title:
Intervention with Schistosoma japonicum cysteine protease inhibitor for treatment of lipopolysaccharide-induced sepsis in mice
作者:
万勇坤李徽徽左琳王小莉王黎源贺文欣姜辉王守祥盛洁张敏钱海春杨芳芳谢红 高世芳方强杨小迪刘牧林
关键词:
脓毒症日本血吸虫半胱氨酸蛋白酶抑制剂脂多糖促炎因子免疫调节因子
Keywords:
sepsis Schistosoma japonicum cysteine protease inhibitors lipopolysaccharide proinflammatory cytokines immunoregulatory cytokines
摘要:
目的探讨日本血吸虫半胱氨酸蛋白酶抑制剂(SjCystatin)对脂多糖(LPS)诱导的小鼠脓毒症的干预效果。方法54 只 BALB/c小鼠适应性饲养1 周后,随机分为正常对照组(PBS 组,A组)、脓毒症造模组(PBS+LPS组,B组)、蛋白干预组(PBS+ LPS+SjCystatin组,C组)。A组腹腔注射PBS(100 μL),B组、C组腹腔注射含LPS(10 mg/kg)的PBS(100 μL),其中C组小鼠于 注射LPS后30 min腹腔注射含25 μg SjCystatin蛋白的PBS(100 μL)。每组随机抽取10只,造模后24 h取小鼠血清及肝、肺、肾 组织,酶联免疫吸附试验(ELISA)验检血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)水平,全自 动生化分析仪检测ALT、AST、BUN和Cr水平;肝、肺和肾组织HE染色观察其病理损伤;每组余8只小鼠,造模后记录小鼠72 h 生存率的差别且观察小鼠状态的改变。结果3组小鼠的72 h生存率具有统计学差异(P<0.05),与A组(100%)相比,B组(0%) 72 h生存率降低(P<0.05),经过SjCystatin蛋白治疗后,C组生存率较B组增高(36%)。与A组相比,B组肝、肺、肾组织切片病理 损伤加重,血清中ALT、AST、BUN、Cr、IL-6、TNF-α水平明显升高(P<0.05);与B组相比,C组调节因子IL-10水平明显升高,肝、 肾、肺组织损伤明显减轻,且血清中上述肝肾功能检测指标及促炎因子水平明显降低(P<0.05)。结论SjCystatin对LPS诱导的 脓毒症有显著的治疗作用。
Abstract:
Objective To observe the effect of Schistosoma japonicum cysteine protease inhibitor (rSjCystatin) for treatment of lipopolysaccharide (LPS)-induced sepsis in mice. Methods After a week of adaptive feeding, 54 BALB/c mice were randomly divided into normal control group (group A), sepsis group (group B), and rSjCystatin intervention group (group C). The mice in group A received an intraperitoneal injection of PBS (100 μL), and those in groups B and C were injected with PBS (100 μL) containing LPS (10 mg/kg); the mice in group C were also intraperitoneally injected with 25 μg sjCystatin in 100 μL PBS 30 min after LPS injection. From each group, 10 mice were randomly selected 24 h after PBS or LPS injection for detecting serum levels of TNF-α, IL-6, and IL-10 using ELISA and the levels of ALT, AST, BUN, and Cr using automatic biochemical analyzer; the pathological changes in the liver, lung and kidney were observed with HE staining. The remaining 8 mice in each group were used for observing the changes in the general condition and the 72-h survival. Results The 72-h survival rates of the mice was 100% in group A, 0 in group B, and 36% in group C, showing a significant difference among the 3 groups (P<0.05). Compared with those in group A, the mice in group B exhibited obvious liver, lung, and renal pathologies with increased levels of ALT, AST, BUN, Cr, IL- 6, and TNF-α (P<0.05). Treatment with sjCystatin significantly lessened LPS- induced organ pathologies, lowered the levels of liver and renal functional indexes and the pro-inflammatory cytokines, and increased the serum level of IL-10 in the mice (P<0.05). Conclusion SjCystatin can produce a significant therapeutic effect on sepsis induced by LPS in mice.

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更新日期/Last Update: 1900-01-01