[1]颜秋霞,马义,陈润强,等.弱精子症患者精子中DKKL1的表达[J].南方医科大学学报,2018,(03):324.
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弱精子症患者精子中DKKL1的表达()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2018年03期
页码:
324
栏目:
出版日期:
2018-04-03

文章信息/Info

Title:
Expression of DKKL1 in spermatozoa of men with asthenospermia
作者:
颜秋霞马义陈润强周秀琴乔静冼英杰冯玲陈彩蓉
关键词:
DKKL1男性不育弱子精症
Keywords:
DKKL1male infertility asthenospermia
摘要:
目的探讨精子顶体相关基因(DKKL1)在弱精子症患者精子中的表达特征及临床意义。方法收集正常男性和弱精子症 患者精液,各取10 μL精液用全自动计算机辅助精液分析系统(CASA)对精液进行常规分析。为了避免白细胞和生精细胞对结 果的影响,剩余精液采用4个梯度的Percoll法离心分离和纯化精子。Trizol法提取精子RNA,实时荧光定量PCR从基因水平检 测DKKL1基因在正常人和弱精子症患者精子中的表达差异;最后采用Western blot方法从蛋白水平检测DKKL1蛋白在正常人 和弱精子症患者精子中的表达差异。结果精液常规分析结果显示,弱精子症和正常人两组之间的年龄、精液体积、精子浓度和 正常精子形态比例均没有统计学差异(P>0.05)。与正常对照组相比,弱精子症组PR 和PR+NP 精子的比例显著降低(P< 0.01)。实时荧光定量PCR结果显示,与正常对照组相比,DKKL1 mRNA在弱精子症患者精子中的表达量降低11.1倍。此外, Western blot结果显示,弱精子症组DKKL1蛋白的表达量与正常对照组相比,降低了2.4倍,差异具有统计学意义(P<0.01),该 结果与实时荧光定量PCR结果一致。结论DKKL1在弱精子症患者精子中的表达水平明显下降,表明其与精子运动密切相关, 是导致弱精子症发生的原因之一。
Abstract:
Objective To compare the expression of DKKL1 in ejaculated spermatozoa of normal fertile men and men with asthenospermia and investigate the role of DKKL1 in the pathogenesis of asthenospermia. Methods The characteristics of semen samples collected from normal fertile men and men with asthenospermia were analyzed using computer-assisted sperm analysis according to WHO criteria. The ejaculated sperms were isolated by Percoll discontinuous density gradients to detect the expression of DKKL1 mRNA and protein using real-time PCR and Western blotting. Results The expression of DKKL1 mRNA was significantly down-regulated by 11.1 times in asthenospermic men as compared with that in normal fertile men (P<0.01). Western blotting showed that the expression of DKKL1 protein was down-regulated by 2.4 times in asthenospermic men compared to normal fertile men. Conclusion The expression of DKKL1, which may play an important role in sperm motility,is significantly decreased in ejaculated spermatozoa of men with asthenospermia.
更新日期/Last Update: 1900-01-01