[1]唐华均,杨欢,范艺巾,等.低强度脉冲超声对环磷酰胺诱导的大鼠卵巢损伤有修复作用[J].南方医科大学学报,2017,(12):1632.
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低强度脉冲超声对环磷酰胺诱导的大鼠卵巢损伤有修复作用()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2017年12期
页码:
1632
栏目:
出版日期:
2017-12-20

文章信息/Info

Title:
Low-intensity pulsed ultrasound promotes repair of cyclophosphamide-induced ovarian injury in rats
作者:
唐华均杨欢范艺巾李成志
关键词:
低强度脉冲超声环磷酰胺卵巢损伤卵巢早衰
Keywords:
low-intensity pulsed ultrasound cyclophosphamide damage of ovarian function premature ovarian failure
摘要:
目的探讨低强度脉冲超声对腹腔注射环磷酰胺引起的SD大鼠卵巢损伤的修复作用。方法63只清洁级雌性SD大鼠随 机取13只为正常组,其余50只连续5 d腹腔注射环磷酰胺30 mg/kg,建立卵巢早衰动物模型。将造模成功的43只大鼠随机分 为实验对照组(21只)、治疗组(22只)。正常组、实验对照组不干预;治疗组给予低强度脉冲超声辐照。监测各组大鼠动情周期 的变化,辐照结束后7 d处死大鼠,测定血清雌二醇(E2),卵泡刺激素(FSH),抗苗勒管激素(AMH)的变化,观察各组大鼠卵巢形 态学及卵泡数量变化。结果注射环磷酰胺后,与正常组相比,实验对照组动情周期紊乱,各级卵泡数明显减少,血清E2和AMH 显著降低,FSH显著升高(P=0.01);超声辐照后,与实验对照组相比,治疗组动情周期恢复正常,卵泡数明显增加,血清E2显著增 高(P=0.01),FSH显著降低(P=0.01),AMH变化不明显(P=0.50)。结论低强度脉冲超声对腹腔注射环磷酰胺引起的SD大鼠 卵巢的损伤有修复作用。
Abstract:
Objective To study the effects of low-intensity pulsed ultrasound (LIPUS) in promoting repair of cyclophosphamide (CTX)-induced ovarian injury in female rats. Methods Fifty female SD rats were subjected to intraperitoneal injection of cyclophosphamide at 30 mg/kg for 5 consecutive days to induce premature ovarian failure. Successful modeling was achieved in 43 rats, which were then randomized into model group (21 rats) and treatment group (22 rats), with another 13 rats without any injections serving as the normal control group. The rats in the treatment group received low-intensity pulsed ultrasound treatment, and those in the other two groups received no interventions. The estrous cycle of the rats was monitored during the experiment, and all the rats were sacrificed 7 days after the treatment for analysis of serum estradiol (E2), follicle stimulating hormone (FSH), anti-Mullerian hormone (AMH), morphological changes of the ovary and the number of follicles. Results Compared with those in the normal control group, the rats in the model group showed disorders in the estrous cycle, reduced number of follicles at all levels, significantly decreased serum E2 and AMH, and increased serum FSH following CTX injection (P=0.01). Ultrasound treatment obviously promoted the recovery of estrous cycle, significantly increased the number of follicles and serum E2 (P=0.01), and decreased FSH (P=0.01) in the rats with CTX-induced ovarian injury, but AMH showed no significant changes after ultrasound treatment (P=0.50). Conclusion Low-intensity pulsed ultrasound can promote the repair of the CTX-induced ovarian injury in SD rats.

相似文献/References:

[1]梁利霞,晁岚,邓晓惠,等.小鼠骨髓移植修复卵巢功能损伤[J].南方医科大学学报,2011,(09):1534.
[2]彭萍,吴璇,关婷,等.1-磷酸-神经鞘氨醇干预化疗对荷瘤小鼠卵巢功能和抑瘤效果的影响[J].南方医科大学学报,2012,(03):383.
[3]冯晨,唐锁勤,王建文,等.Topotecan plus cyclophosphamide as maintenance chemotherapy for children with high-risk neuroblastoma in complete remission: short-term curative effects and toxicity[J].南方医科大学学报,2013,(08):1107.
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更新日期/Last Update: 1900-01-01