[1]付绍杰,奉艳林,于立新,等.等候肾移植患者中RHD阴性血液样品的基因分型及意义[J].南方医科大学学报,2017,(06):837.
点击复制

等候肾移植患者中RHD阴性血液样品的基因分型及意义()
分享到:

《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2017年06期
页码:
837
栏目:
出版日期:
2017-06-20

文章信息/Info

Title:
Genotyping of RhD-negative blood samples diagnosed by serological tests from patients waiting for kidney transplantation
作者:
付绍杰奉艳林于立新苗芸罗敏王亦斌李怿辰陈树汉肖露露
关键词:
RhD血型阴性肾移植分子生物学
Keywords:
RhD blood group kidney transplantation polymerase chain reaction real time
摘要:
目的探讨等候肾移植患者中RHD阴性血型基因血清学与分子生物学的差异。方法收集2006年1月~2016年1月广州 市各器官移植中心等候肾移植的患者中经血清学检测为RhD阴性的血样103例,采用分子生物学方法(定量PCR-测序技术)进 行RHD基因分型。血清学与分子生物学两种方法对RhD假阴性检出率行χ2检验。结果103例血样中,RhD真阴性(即10个外 显子全部缺失)56例(54.5%),RhD假阴性(即RhD阳性,但是10个外显子中有部分缺失、重复、错义突变)47例(45.6%)。在47 例RhD假阴性中,弱D(weak D)1例(2.1%)、部分D(partial D)13例(27.7%)、放散D(D-elution)33例(70.2%)。血清学与分子生 物学对RhD阴性识别的差异呈统计学显著性(P<0.05)。结论103例血清学检测出的RhD阴性中有45.6%通过分子生物学方 法检测实际为RhD阳性变异体,说明血清学技术对识别RhD阴性呈较高错误率。利用分子生物学技术进行受者和供者RHD 血型基因分型,对于精确肾移植配型具有重要的临床意义。
Abstract:
Objective To compare the accuracy of serological and molecular approaches to identification of RhD-negative patients waiting for kidney transplantation. Methods A total of 103 RhD-negative blood samples by serological test were collected from patients waiting for kidney transplantation between January, 2006 and January, 2016. Quantitative PCR and sequencing were used to verify the results of RHD genotyping, and the false negative rates of the serological and molecular methods for RhD genotyping were compared. Results Among the 103 blood samples, true RhD negativity (with all the 10 exons missing) was found in 56 samples (54.5%), and false RhD negativity (RhD positivity with loss, repetition, or missense mutation in the 10 exons) in 47 samples (45.6%). In the 47 false RhD-negative cases, weak D was detected in 1 case (2.1%), partial D in 13 cases (27.7%), and D-elution in 33 cases (70.2%). The detection rates of RhD negativity differed significantly between the serological and molecular methods (P<0.05). Conclusion Serological test is associated with a high false negative rate in detecting RhD blood group, and the use of the molecular approach has important clinical significance in accurate RhD genotyping for patients waiting for renal transplantation.

相似文献/References:

[1]刘丁,蔡绍曦,刘永光,等.肾移植术后侵袭性肺部真菌感染的预后因素分析——单中心10年经验回顾性分析[J].南方医科大学学报,2011,(05):882.
[2]黄君,吴英,苏泽轩,等.移植肾术后早期肾动脉阻力指数与血肌酐值的相关性分析[J].南方医科大学学报,2011,(06):1105.
[3]司中洲,李亭,李杰群,等.白细胞介素-17 与Th17 细胞在小鼠肾移植急性排斥反应中的表达及意义[J].南方医科大学学报,2011,(08):1374.
[4]石向华,刘小友,孙煦勇,等.CD40 阻断剂对大鼠肾移植急性排斥反应的影响[J].南方医科大学学报,2011,(12):2085.
[5]于立新,熊海云,付绍杰,等.移植肾动脉狭窄危险因素对比研究[J].南方医科大学学报,2006,(08):1160.
 YU Li-xin,XIONG Hai-yun,FU Shao-jie,et al.Retrospective study of the risk factors of transplant renal artery stenosis[J].,2006,(06):1160.
[6]王平贤,范明齐,黄赤兵,等.转化生长因子β1对远期移植肾功能影响的临床观察[J].南方医科大学学报,2006,(09):1352.
 WANG Ping-xian,FAN Ming-qi,HUANG Chi-bing,et al.Influence of transforming growth factor β1 on long-term renal allograft function[J].,2006,(06):1352.
[7]朱云松,徐安平,何恢绪,等.单剂半量赛尼哌预防移植肾急性排斥的临床观察[J].南方医科大学学报,2006,(12):1818.
 ZHU Yun-song,XU An-ping,HE Huei-xu,et al.Half-dose Zenapax for acute rejection prevention after renal transplantation[J].,2006,(06):1818.
[8]聂海波,何恢绪,李逊,等.肾移植术后新尿路梗阻的临床处理(附16例报告)[J].南方医科大学学报,2005,(11):1454.
 NIE Hai-bo,HE Hui-xu,LI Xun,et al.Management of urinary obstruction following renal transplantation:report of 16 cases[J].,2005,(06):1454.
[9]于立新,王志鹏,付绍杰,等.丙型肝炎病毒感染对肾移植术后影响的临床研究[J].南方医科大学学报,2004,(06):682.
 YU Li-xin,WANG Zhi-peng,FU Shao-jie,et al.Hepatitis C virus infection and clinical outcome of kidney transplantation[J].,2004,(06):682.
[10]黄先恩,范礼佩,李留洋,等.致敏受者肾移植急性排斥反应的影响因素[J].南方医科大学学报,2004,(10):1188.
 HUANG Xian-en,FAN Li-pei,LI Liu-yang,et al.Risk factors of acute rejection in sensitized kidney transplant recipients[J].,2004,(06):1188.

更新日期/Last Update: 1900-01-01