[1]麦汉滔,姜涛,张爱武,等.小鼠脑梗死后脑组织HDAC9的表达及其意义[J].南方医科大学学报,2017,(06):812.
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小鼠脑梗死后脑组织HDAC9的表达及其意义()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2017年06期
页码:
812
栏目:
出版日期:
2017-06-20

文章信息/Info

Title:
Expression of HDAC9 in different brain regions in mice with cerebral ischemic stroke
作者:
麦汉滔姜涛张爱武吕田明杨灿洪覃偲偲
关键词:
脑卒中组蛋白去乙酰化酶线栓法大脑中动脉阻塞模型亚细胞定位
Keywords:
ischemic stroke histone deacetylase middle cerebral artery occlusion subcellular localization
摘要:
目的检测线栓法大脑中动脉阻塞(MCAO)后小鼠不同部位脑组织HDCA9的表达及胞内分布变化,探讨HDAC9与缺血 性脑卒中的关系。方法21只雄性C57BL/6小鼠随机分为假手术组(9只)与手术组(12只),将手术组术后Longa评分2~3 分的 小鼠纳入MCAO组(9只),术后3 d断头取脑,采用免疫荧光染色观察HDAC9脑组织内分布位置与胞内分布变化,采用Western 免疫印迹法与实时荧光定量PCR法检测各组不同部位(梗死侧/对侧皮层/MCAO组小脑/假手术组皮层/假手术组小脑)HDAC9 的表达变化。结果(1)免疫荧光染色:脑梗死后,梗死灶周围脑组织HDAC9的荧光强度较其他部位明显增强。高倍镜下与假 手术组对比,梗死灶周围HDAC9 胞浆内表达增多,核内表达减少;(2)蛋白与mRNA表达检测:与各组对比,梗死侧脑组织 HDAC9 表达显著升高,具有统计学差异(均P<0.05),蛋白与mRNA表达情况相一致。结论HDAC9 与缺血性脑卒中密切相 关,可能参与其病理生理过程。
Abstract:
Objective To investigate the expression and the subcellular localization of HDAC9 in different brain regions of mice after cerebral ischemic injury and explore the association between HDAC9 and ischemic stroke. Methods Twenty-one male C57BL/6 mice were randomly divided into sham-operated group (n=9) and operated group (n=12). In the latter group, the mice with Zea-Longa neurological deficit scores of 2 or 3 following middle cerebral artery occlusion (MCAO) were assigned into MCAO group (n=9). Immunofluorescence was performed to investigate the subcellular localization of HDAC9 in the brain tissues on day 3 after MCAO. Western blotting and qRT-PCR were used to analyze the expression of HDAC9 in different regions of the brain. Results Immunofluorescence showed more intense HDAC9 expressions in the brain tissues around the infarct focus, and in the cells surrounding the infarct, HDAC9 expression was obviously increased in the cytoplasm and reduced in the cell nuclei. Compared with the other brain regions, the ipsilesional cortex with MCAO showed more abundant HDAC9 expressions at both the mRNA and protein levels (P<0.05). Conclusion HDAC9 may be closely related to cerebral ischemic injury and participate in the pathophysiological process of ischemic stroke.

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更新日期/Last Update: 1900-01-01