[1]陈璇,许晓虎,吴昕彧,等.变异链球菌UA159中SMU.2055基因在致龋性的作用[J].南方医科大学学报,2017,(06):786.
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变异链球菌UA159中SMU.2055基因在致龋性的作用()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2017年06期
页码:
786
栏目:
出版日期:
2017-06-20

文章信息/Info

Title:
Role of SMU.2055 gene in cariogenic capacity of Streptococcus mutans
作者:
陈璇许晓虎吴昕彧李转玲赵望泓
关键词:
变异链球菌乙酰基转移酶SMU.2055基因致龋性
Keywords:
Streptococcus mutans acetylase SMU.2055 gene cariogenic
摘要:
目的构建变异链球菌(Streptococcus mutans, S. mutans)SMU.2055基因缺陷菌株,确定SMU.2055基因对S.mutans形态及 与致龋性相关的生物学特性的影响。方法利用同源重组的方法,构建SMU.2055基因缺陷菌株。在扫描电子显微镜及透射电 子显微镜下观察野生菌株及基因缺陷菌株的形态结构。连续监测野生菌株及基因缺陷菌株的菌液A600值及pH值,比较其生长 能力及产酸能力。对两种菌株进行酸适应后,分别进行酸冲击,评价其耐酸能力。结果经过聚合酶链反应及测序鉴定,变异链 球菌SMU.2055基因缺陷菌株构建成功。扫描电子显微镜结果显示,与野生菌株相比,基因缺陷菌株菌体形态发生改变,菌体 间不规则物质较少。透射电子显微镜下观察,基因缺陷菌株细胞膜较模糊,细胞膜完整性被破坏。基因缺陷菌株的生长能力及 耐酸能力较野生菌株均有所下降,而基因缺陷菌株与野生菌株间的产酸能力未见明显差异。结论SMU.2055基因与变异链球 菌的形态、生长及耐酸能力密切相关,而对其产酸能力不造成明显影响。
Abstract:
Objective To construct a SMU.2055-dificient mutant strain of Streptococcus mutans (S. mutans) and evaluate its cariogenic capacity in comparison with wild-type S. mutans. Methods The SMU.2055-dificient mutant strain of S. mutans was constructed using homologous recombination technique and observed with scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The absorbance at 600 nm and pH values of the wild-type and mutant strains were monitored to evaluate their growth and acid production. After acid adaption, the two strains were challenged with acid shock and their survival rates were determined. Results PCR and sequence analyses verified the successful construction of the SMU.2055-dificient mutant strain. Observation with SEM revealed obvious changes in the morphology of the mutant strain, which showed reduced irregular substances between the individual bacteria as compared with the wild-type strain. TEM revealed major alterations in the cellular architecture of the mutant strain with blurry cell membrane and disruption of the membrane integrity. The growth capacity of the mutant strain decreased in both normal and acidic conditions but its acid production capacity remained unaffected. Conclusion SMU.2055 gene is associated with morphology maintenance, growth capacity and acid resistance of S. mutans but is not related to the acid production capacity of the bacterium.

相似文献/References:

[1]胡桐楠,郑伟,李少华,等.不同pH条件下高低致龋性变异链球菌sRNA SpR19及其潜在靶标GroEL的表达变化[J].南方医科大学学报,2017,(06):802.

更新日期/Last Update: 1900-01-01