[1]吴春艳,张辉见,范存霞,等.促肾上腺皮质激素非依赖性库欣综合征患者肾周及皮下脂肪组织的炎症及纤维化[J].南方医科大学学报,2017,(04):563.
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促肾上腺皮质激素非依赖性库欣综合征患者肾周及皮下脂肪组织的炎症及纤维化()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2017年04期
页码:
563
栏目:
出版日期:
2017-04-20

文章信息/Info

Title:
Expressions of inflammatory and fibrogenic factors in perinephric and subcutaneous adipose tissues of patients with adrenocorticotropic hormone-independent Cushing’s syndrome
作者:
吴春艳张辉见范存霞吴芃魏强蔡迎迎邹少洲王玲薛耀明关美萍
关键词:
库欣综合征脂肪组织炎症纤维化
Keywords:
Cushing’s syndrome adipose tissues inflammation fibrosis
摘要:
目的探讨促肾上腺皮质激素(ACTH)非依赖性库欣综合征患者不同部位脂肪组织炎症及纤维化程度。方法收集行后 腹腔镜下肾上腺肿物切除术的ACTH非依赖性库欣综合征患者的肾脏周围及皮下脂肪组织,RT-PCR检测白介素-6(IL-6)、肿瘤 坏死因子-α(TNF-α)及基质金属蛋白酶-2(MMP-2)、金属蛋白酶类组织抑制剂1(TIMP1)、早期生长反应因子1(EGR1)及脂肪 因子CCAAT增强子结合蛋白β(CEBPβ)、解耦联蛋白-1(UCP-1)、过氧化物酶体增殖物激活受体γ共激活因子1α(PGC1α)及细 胞死亡介导的DNA碎片因子样受体a(CIDEA)mRNA的表达。结果ACTH 非依赖性库欣综合征患者肾周脂肪组织中 CIDEA mRNA的表达显著高于皮下脂肪组织(P<0.05)。而脂肪因子CEBPβ、UCP-1及PGC1α mRNA的表达在肾脏周围脂肪 及皮下脂肪组织中差异无统计学意义。皮下脂肪组织IL-6、TIMP1 及EGR1 mRNA的表达显著高于肾脏周围脂肪组织(P< 0.05)。肾周脂肪组织及皮下脂肪中TNF-α及MMP-2 mRNA的表达相似。结论库欣综合征患者的皮下脂肪组织中的炎症及 纤维化程度高于肾脏周围脂肪。提示不同部位脂肪组织具有不同的生物学特性,长时间较高水平的皮质醇刺激可能导致皮下 脂肪炎症。
Abstract:
Objective To investigate the expressions of inflammation- and fibrosis-related genes in perinephric and subcutaneous adipose tissues in patients with adrenocorticotropic hormone (ACTH)-independent Cushing’s syndrome. Methods The perinephric and subcutaneous adipose tissues adipose tissues were obtained from 8 patients with ACTH-independent Cushing’s syndrome undergoing laparoscopic retroperitoneal adrenalectomy. Real-time PCR was used to detect the mRNA expression levels of interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), matrix metallopeptidase 2 (MMP-2), TIMP metallopeptidase inhibitor 1 (TIMP-1), early growth response 1 (EGR1), CCAAT/enhancer binding protein β (CEBPβ), uncoupling protein 1(UCP-1), PPARγ coactivator 1 alpha (PGC1α) and cell death-inducing DFFA-like effector a (CIDEA). Results The mRNA level of CIDEA was significantly higher in the perinephric adipose tissue (peri-N) than in the subcutaneous adipose tissue (subQ) (P<0.05). The expressions of CEBPβ, UCP-1, and PGC1α mRNA in the peri-N were similar with those in the subQ. The expressions of IL-6, TIMP1 and EGR1 mRNA in the subQ were significantly higher than those in the peri-N (P<0.05). No significant difference in TNF-α and MMP-2 mRNA levels was found between peri-N and subQ. Conclusion The expression levels of the inflammation- and fibrosis-related genes are higher in the subQ than in the peri-N of patients with ACTH-independent Cushing’s syndrome, suggesting that chronic exposure to endogenous hypercortisolism may cause adipose tissue dysfunction.

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更新日期/Last Update: 1900-01-01