[1]周芳竹,王欣,代安亚,等.藏药桃儿七可促进慢性粒细胞白血病K562细胞的凋亡[J].南方医科大学学报,2017,(02):226.
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藏药桃儿七可促进慢性粒细胞白血病K562细胞的凋亡()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2017年02期
页码:
226
栏目:
出版日期:
2017-01-22

文章信息/Info

Title:
Effects of Sinopodophyllum hexundrum on apoptosis in K562 cells
作者:
周芳竹王欣代安亚黄峥兰李会黄宁姝冯文莉
关键词:
藏药桃儿七慢性粒细胞白血病BCR/ABLSTAT5信号通路凋亡
Keywords:
Sinopodophyllum hexundrum chronic myeloid leukemia BCR/ABL STAT5 signal pathways cell apoptosis
摘要:
目的探讨藏药桃儿七对慢性粒细胞白血病K562细胞凋亡的影响及相关机制。方法用不同浓度的桃儿七对K562细胞 株进行不同时间梯度的处理,应用CCK8试验筛选桃儿七的最佳作用浓度及时间;流式细胞术检测细胞凋亡;瑞氏染色观察细 胞形态学改变,DAPI染色观察细胞核形态变化;Western blotting分别检测凋亡相关蛋白PARP、caspase-3、Cleaved-caspase3的 活化情况以及BCR/ABL、p-BCR/ABL、STAT5、p-STAT5蛋白表达变化。结果分别以1、2、3 μg/mL浓度的桃儿七处理细胞12、 24、36、48、72、96 h,K562细胞增殖呈浓度时间依赖性抑制,并筛选出2 μg/mL,48 h为最佳处理方式。流式细胞术检测结果显示 凋亡细胞数量呈时间依赖性增加,并在48 h凋亡最为显著;凋亡相关蛋白PARP、caspase-3以及Cleaved-caspase-3均呈时间依赖 性活化。以2 μg/mL 桃儿七处理细胞48 h 后,K562 细胞形态发生不规则改变,出现核碎裂、溶解等凋亡特征;BCR/ABL、 p-BCR/ABL、STAT5、p-STAT5表达显著降低。结论藏药桃儿七能够促进慢性粒细胞白血病K562细胞凋亡,其机制可能通过 抑制BCR/ABL-STAT5信号通路并激活线粒体凋亡途径来实现。
Abstract:
Objective To investigate the effects of Sinopodophyllum hexundrum on apoptosis in K562 cells. Methods K562 cells were treated with Sinopodophyllum hexundrum at different concentrations and for different lengths of time to determine the optimal conditions of SinoPodophyllum hexandrum treatment for K562 cells using CCK8 assay. The cell apoptotic rate was detected by flow cytometry, and the cell morphology and nuclear morphology of K562 cells were observed with Wright staining and DPAI staining, respectively. The protein expressions of BCR/ABL, p-BCR/ABL, STAT5, p-STAT5 and the apoptosis-related proteins PARP, caspase-3 and cleaved-caspase-3 were determined with Western blotting. Results The cell proliferation was inhibited in a concentration-and time-dependent manner by 1, 2, and 3 μg/mL Sinopodophyllum hexundrum. The treatment was optimal with a Sinopodophyllum hexundrum concentration of 2 μg/mL a treatment time of 48 h, and the cell apoptotic rate increased in a time-dependent manner and significantly increased at 48 h (P<0.001). The expression of apoptosis-related proteins PARP, caspase-3 and cleaved-caspase-3 were also activated in a time-dependent manner. The cells showed typical apoptotic changes after treatment with 2 μg/mL Sinopodophyllum hexundrum for 48 h with significantly reduced expressions of BCR/ABL, p-BCR/ABL, STAT5, AND p-STAT5. Conclusion Sinopodophyllum hexundrum promotes K562 cell apoptosis possibly by inhibiting BCR/ABL-STAT5 survival signal pathways and activating the mitochondrion-associated apoptotic pathways.

相似文献/References:

[1]刘张玲,胡晶,黄峥兰,等.吲哚美辛对K562细胞株BCR/ABL-Wnt/β-catenin信号通路的影响[J].南方医科大学学报,2015,(07):998.

更新日期/Last Update: 1900-01-01