[1]曹罗元,杨菁,富显果,等.miR-205通过下调ZEB1和ZEB2表达抑制肾小管上皮细胞转分化[J].南方医科大学学报,2016,(12):1700.
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miR-205通过下调ZEB1和ZEB2表达抑制肾小管上皮细胞转分化()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2016年12期
页码:
1700
栏目:
出版日期:
2016-12-20

文章信息/Info

Title:
The MicroRNA miR-205 inhibits epithelial-messenchymal transition in HK-2 cells by down-regulating ZEB1 and ZEB2 expressions
作者:
曹罗元杨菁富显果林应华林峰黄宝英
关键词:
miR-205转分化HK-2 细胞株ZEB1
Keywords:
miR-205 epithelial-messenchymal transition HK-2 cells ZEB1
摘要:
目的探讨miR-205在肾小管上皮细胞转分化的作用机制。方法将miR-205 mimics和scrambled control分别转染HK-2 转分化细胞株,采用real-time qPCR检测miR-205 和ZEB1、E-cadherin、α-SMA mRNA的表达水平;运用Western blotting 检测 ZEB1、ZEB2、E-cadherin、α-SMA的表达水平。通过细胞免疫组化检测β-catenin的异位表达情况和E-cadherin的表达情况。结 果miR-205 mimics 转染HK-2转分化细胞株后,ZEB1和ZEB2的表达水平较高糖组得到大幅下调(P<0.01),而E-cadherin的 表达水平较高糖组显著提高(P<0.01),同时间质细胞特征分子α-SMA的表达水平显著降低(P<0.01)。miR-205 mimics 也显著 抑制了HK-2 转分化过程中β-catenin 异位表达,在维持上皮细胞的形态方面也起着重要的作用。结论miR-205 可通过下调 ZEB1和ZEB2的表达,抑制了肾小管上皮间质转分化进程。
Abstract:
Objective To explore the role of miR-205 in regulating epithelial-messenchymal transition (EMT) in proximal tubular cell line HK-2 cells and the underlying mechanism. Methods HK-2 cells transfected with miR-205 mimics or a scrambled control sequence were examined for miR-205 expressions and mRNA levels of ZEB1, E-cadherin, and α-SMA using real-time qPCR; the protein levels of ZEB1, ZEB2, E-cadherin, and α-SMA were detected with Western blotting. Immunohistochemistry was performed to examine the ectopic expression of β-catenin and E-cadherin expression in the cells. Results The expression levels of ZEB1 and ZEB2 decreased significantly (P<0.01) while E-cadherin expression was up-regulated (P<0.01) in cells transfected with miR-205 mimics. Transfection with miR-205 mimics also markedly down-regulated the expression of α-SMA (P<0.01), a marker of mesenchymal cells that play an important role in EMT of HK-2 cells. The ectopic expression of β-catenin was inhibited by miR-205 mimics in HK-2 cells. Conclusion miR-205 inhibits EMT in HK-2 cells by down-regulating the expression levels of ZEB1 and ZEB2.
更新日期/Last Update: 1900-01-01