[1]巫剑峰,董淑英,武丹丹,等.Pannexin1通道在顺铂诱导睾丸癌I-10细胞凋亡中的作用[J].南方医科大学学报,2016,(11):1456.
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Pannexin1通道在顺铂诱导睾丸癌I-10细胞凋亡中的作用()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2016年11期
页码:
1456
栏目:
出版日期:
2016-11-20

文章信息/Info

Title:
Role of pannexin 1 channels in cisplatin-induced apoptosis in I-10 cells
作者:
巫剑峰董淑英武丹丹陈银玲童旭辉
关键词:
甘珀酸pannexin1通道顺铂凋亡ATP睾丸癌
Keywords:
carbenoxolone pannexin1 channel cisplatin apoptosis ATP testiculus carcinoma
摘要:
目的观察pannexin1通道在顺铂诱导睾丸癌I-10细胞凋亡中的作用及其机制。方法MTT法检测细胞存活率;Annexin V/PI双染法和Hoechst 33258染色法分别检测细胞早期和晚期凋亡;化学发光法检测细胞外ATP浓度;ELISA法检测细胞内IP3 含量。结果MTT法显示pannexin1通道抑制剂CBX与顺铂合用组的细胞存活率高于单用顺铂组(P<0.01);Annexin V/PI双染 法显示CBX与顺铂合用组的细胞早期凋亡率低于单用顺铂组(P<0.001);Hoechst 33258染色法显示CBX与顺铂合用组的细胞 晚期凋亡率低于单用顺铂组(P<0.01);化学发光法表明CBX与顺铂合用组的细胞外ATP浓度低于单用顺铂组(P<0.05);ELISA 法表明CBX与顺铂合用组的细胞内IP3浓度低于单用顺铂组(P<0.05)。结论Pannexin1通道参与顺铂诱导睾丸癌I-10细胞的 凋亡,其机制可能与介导ATP/IP3信号通路有关。
Abstract:
Objective To investigate the role of pannexin 1 channels in cisplatin-induced apoptosis in I-10 cells and the mechanisms. Methods MTT assay was used to assess the cytotoxicity of cisplatin (DDP) in I-10 cells. Annexin V/PI double staining and Hoechst 33258 fluorescence staining were employed to detect early- and late-stage apoptosis of the cells, respectively. Extracellular ATP level and intracellular IP3 level in the cells were detected using commercial detection kits. Results I-10 cells exposed to both CBX (a pannexin 1 channel inhibitor) and DDP showed a higher cell viability compared with the cells exposed to DDP alone (P<0.01). CBX significantly decreased cisplatin-induced early-stage apoptosis (P<0.001) and late-stage apoptosis (P<0.01), and cause obvious reductions in extracellular ATP and intracellular IP3 levels during cisplatin-induced apoptosis (P<0.05). Conclusion Pannexin 1 channels participate in cisplatin-induced apoptosis in I-10 cells possibly through the ATP/IP3 pathway.
更新日期/Last Update: 1900-01-01