[1]李良庆,谢文焌,潘敦.巴弗洛霉素A1抑制胃癌MGC-803细胞增殖及增强奥沙利铂的敏感性[J].南方医科大学学报,2015,(10):1400.
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巴弗洛霉素A1抑制胃癌MGC-803细胞增殖及增强奥沙利铂的敏感性()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2015年10期
页码:
1400
栏目:
出版日期:
2015-10-20

文章信息/Info

Title:
Effect of bafilomycin A1 on proliferation and oxaliplatin sensitivity in gastric cancer
MGC-803 cells
作者:
李良庆谢文焌潘敦
关键词:
巴弗洛霉素A1自噬奥沙利铂凋亡侵袭能力
Keywords:
bafilomycin A1 autophagy oxaliplatin apoptosis invasive ability
摘要:
目的探讨巴弗洛霉素A1对抑制胃癌细胞自噬、增殖、侵袭、凋亡和奥沙利铂敏感性的影响。方法将胃癌MGC-803细胞
分为空白对照组(Control组)、巴弗洛霉素A1组(BAF组)、奥沙利铂组(OXA组)及巴弗洛霉素A1+奥沙利铂(O+B组)。分别采
用MTT法、平板克隆形成实验检测细胞增殖活力;Elisa法检测细胞核小体表达水平;Transwell法检测细胞运动能力;扫描电
镜、免疫组化及Western blot检测自噬水平;Western blot检测凋亡相关蛋白Bcl-2、Bax表达情况。结果BAF组与Control组相
比自噬水平显著降低,BAF组增殖活力、迁移细胞数及侵袭细胞数显著低于Control组(P=0.000),核小体和Bax表达显著高于
Control组(P=0.000),Bcl-2表达低于Control组(P=0.046)。O+B组与OXA组相比自噬水平显著降低,此时O+B组细胞增殖活
力、迁移细胞数及侵袭细胞数显著低于OXA组(P=0.000),凋亡率显著高于OXA组(P=0.000),Bcl-2相对表达量显著低于OXA
组(P=0.001),Bax相对表达量显著高于OXA组(P=0.000)。结论巴弗洛霉素A1通过抑制自噬能够显著抑制胃癌MGC-803细
胞的增殖和运动能力,促进凋亡,而且能增强奥沙利铂的药物敏感性。
Abstract:
Objective To investigate the effect of bafilomycin A1 (BAF) on the cell proliferation, invasiveness, apoptosis, and
oxaliplatin sensitivity in gastric cancer MGC-803 cells. Methods MGC-803 cells were divided into control group, BAF group,
oxaliplatin group, and BAF+ oxaliplatin group. MTT assay and plate clone formation assay were used to assess the viability
and colony forming ability of the cells after the treatments. The expression of nucleosomes in the cells was examined with
ELISA. The cell migration and invasion after the treatments were evaluated. Western blotting was performed to detect the
expression of Bcl-2 and Bax in the treated cells, and scanning electron microscopy, immunohistochemistry and Western
blotting were employed to to observe the cell autophagy. Results Compared with the control cells, the cells treated with BAF
showed a substantial decrease in autophagosome accumulation with attenuated cell proliferation, migration and invasion.
Compared with cells treated with oxaliplatin alone, the cells treated with both BAF and oxaliplatin showed significantly
lowered autophagosome accumulation, suppressed cell proliferation, migration and invasion, increased cell apoptosis,
increased Bax expression and lowered Bcl-2 expression. Conclusion BAF can inhibit the proliferation and invasiveness of
MGC-803 cells, promote cell apoptosis by inhibiting autophagy, and enhances the sensitivity of the cells to oxaliplatin.

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更新日期/Last Update: 1900-01-01