[1]吴恙,谢李华,刘培文,等.蚊虫的组学研究:媒介生物学和传播疾病的大数据分析平台[J].南方医科大学学报,2015,(05):625.
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蚊虫的组学研究:媒介生物学和传播疾病的大数据分析平台()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2015年05期
页码:
625
栏目:
出版日期:
2015-05-15

文章信息/Info

Title:
Omics of vector mosquitoes:a big data platform for vector biology and vector-borne
diseases
作者:
吴恙谢李华刘培文李小聪闫桂云陈晓光
关键词:
媒介基因组学转录组学小RNA组学蚊媒传染病
Keywords:
mosquito vector genomics transcriptomics small RNAomics vector-borne disease
摘要:
近年来随着二代测序等生物技术的的快速发展,蚊虫基因组学、转录组学、小RNA组学等领域的研究也得到了迅速提
升。迄今为止,已有多种重要蚊媒包括冈比亚按蚊、致倦库蚊、埃及伊蚊等的基因组获得解析;不同蚊种的基因组大小差异很
大,且与基因组中的重复序列的多少呈正相关;基因组序列为嗅觉相关基因、性别决定基因等蚊虫基因的克隆鉴定提供了便
利。转录组研究则给蚊虫基因的功能分析带来了有效手段,吸血蚊虫的分子基础、唾液腺蛋白的分类构成以及滞育的发生机制
等都借此取得了突破性发展。小RNA组研究则揭示miRNA和piRNA对蚊虫的卵巢发育和吸血消化具有调节作用,而且在蚊媒抗病
毒免疫中起重要作用。总之,蚊虫的组学研究为其媒介生物学和传播疾病的防治,提供了广阔的、实用的大数据分析平台。
Abstract:
Recently the studies on mosquito genomics, transcriptomics and small RNAomics developed rapidly with the novel
biotechnologies of the next generation sequencing techniques. The genome sequences of several important vector mosquitoes
including Anopheles gambiae, Culex quinquefasciatus, and Aedes aegypti have been published. The genome sizes vary among the
different species of mosquitoes and are consistent with the number of the repeat regions. The released genome sequences
facilitate gene cloning and identification as for OBP, OR and dsx genes. Transcriptomics provides a useful tool for functional
analyses of the mosquito genes, and using this technique, the molecular basis of mosquito blooding, gland proteins and
diapauses have been explored. Studies on small RNAomics suggest important roles of miRNAs and piRNAs in ovary
development, blood digestion, and immunity against virus infection. The studies on mosquito omics have generated a big data
platform for investigation of vector biology and vector-transmitted disease prevention.
更新日期/Last Update: 1900-01-01