[1]李珉珉,张瑞涛,胡义平,等.HIV进入抑制多肽VIR576可抑制抗原特异性CD4+T细胞的体外活化:一种潜在的双功能杀微生物剂[J].南方医科大学学报,2014,(05):597.
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HIV进入抑制多肽VIR576可抑制抗原特异性CD4+T细胞的体外活化:一种潜在的双功能杀微生物剂()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2014年05期
页码:
597
栏目:
出版日期:
2014-05-15

文章信息/Info

Title:
Peptidic HIV-1 fusion inhibitor VIR576 as a potential dualfunctional
microbicide inhibits antigen-specific CD4 + T-cell
activation
作者:
李珉珉张瑞涛胡义平李建军姜世勃李晓娟刘叔文
关键词:
HIV进入抑制多肽gp41融合多肽VIR576CD4+T细胞活化
Keywords:
HIV-1 fusion inhibitor gp41 fusion peptide VIR576 CD4+T cell activation
摘要:
目的观察VIR576 对CD4+T 细胞活化的作用。方法分离DO11.10 小鼠脾细胞并用OVA或ConA 分别刺激,观察
VIR576对抗原特异性和非特异性T细胞活化的影响;培养A2b CD4+T细胞株和磁珠分离的DO11.10小鼠脾脏CD4+CD25-效应
性T细胞,分别用特异性抗原激活,观察VIR576对抗原特性CD4+T细胞活化的影响。结果VIR576抑制抗原特异性T细胞的
活化,但对非抗原特异性T细胞活化的影响不显著,非特异性T细胞活化不需要TCR和CD3的交联。进一步研究发现,VIR576
也可以下调抗原特异性CD4+T细胞的活化。结论粘膜活化的CD4+T细胞对HIV-1高度易感,VIR576不仅具有抑制HIV进入
的活性还能够抑制CD4+T细胞活化,提示VIR576是一种有开发潜能的预防HIV性传播的杀微生物剂。
Abstract:
Objective To observe if VIR576, an 20-mer peptide derived from the C-proximal subfragment of a1-antitrypsin
(a1-AT) which inhibits human immunodeficiency virus type 1 (HIV-1) entry into the target cells by interacting with fusion
peptide (FP), can also directly inhibit CD4+ T cell activation in vitro. Methods Splenocytes isolated from DO11.10 OVA Tg mice
were stimulated with ovalbumin or concanavalin A to test the effects of VIR576 on antigen-specific or non-antigen-specific T
cell activation. Both primary CD4 +CD25- T cells from DO11.10 mice and CD4 + T cell line A2b were activated with specific
antigens to evaluate the effects of VIR576. Results VIR576 inhibited antigen-specific splenocyte activation but had no
significant effect on non-antigen-specific T-cell activation, which bypassed the crosstalk between the CD3-signaling complex
and TCR. We furthermore observed that VIR576 could also down-regulate antigen-specific CD4+ T-cell activation. Conclusion
Given the high susceptibility of activated CD4+ T cells in the mucosa to HIV-1 infection, the inhibitory effects of VIR576 on both
HIV entry into the target cells and CD4+ T-cell activation suggest the potential of VIR576 as a microbicide for prevention of
sexual transmission of HIV.

相似文献/References:

[1]邵继平,姜世勃,刘叔文.HIV-1 CRF07_BC毒株gp41 NHR结构域N51的表达及结构分析[J].南方医科大学学报,2012,(12):1737.

更新日期/Last Update: 1900-01-01