[1]邓炎尧,侯德仁,田密,等.AD模型小鼠海马和小脑Aβ的沉积与相关miRNAs表达的变化[J].南方医科大学学报,2014,(03):323.
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AD模型小鼠海马和小脑Aβ的沉积与相关miRNAs表达的变化()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2014年03期
页码:
323
栏目:
出版日期:
2014-03-15

文章信息/Info

Title:
β-amyloid peptide deposition and expression of related miRNAs in the cerebellum of a
mouse model of Alzheimer’s disease
作者:
邓炎尧侯德仁田密李维奉夏露
关键词:
阿尔茨海默病淀粉样蛋白microRNA小脑
Keywords:
Alzheimer disease amyloid microRNA cerebellum
摘要:
目的通过对比观察AD模型小鼠海马与小脑Aβ沉积和相关miRNAs表达的变化,探讨AD模型小鼠小脑是否存在Aβ沉
积和相关miRNAs的差异表达。方法选择12月龄大小的APPswe/PS△E9双转基因AD小鼠作为实验组(EG),同月龄的同种
系的野生型小鼠C57为对照组(CG),每组各12只。将小鼠脑组织分为左右两部分,右侧脑组织行刚果红染色检测淀粉样物质
在海马及小脑的沉积;左侧脑组织分别用于提取海马及小脑的miRNA,应用实时荧光定量PCR方法分别检测两组小鼠海马及
小脑组织的miRNA-135a-5p、miRNA-298-5p、miRNA-466b-3p和miR-669f-3p的表达情况。结果刚果红染色:实验组小鼠海马
及小脑均可见橘红色的Aβ沉积,对照组小鼠海马及小脑均未见橘红色的Aβ沉积。实时荧光定量PCR:四种miRNAs在实验组
海马的表达均低于对照组(P<0.05);miRNA-135a-5p、miRNA-298-5p 和miR-669f-3p 在实验组小脑的表达低于对照组(P<
0.05);miRNA-298-5p和miR-669f-3p在实验组海马的表达低于小脑(P<0.05)。结论APPswe/PS△E9双转基因AD小鼠小脑中
也存在Aβ沉积,其形成可能与miRNA-135a-5p、miRNA-298-5p和miR-669f-3p的表达下调有关。
Abstract:
Objective To investigate the presence of β-amyloid peptide (Aβ) deposition in the cerebellum and the expression of
related miRNAs in the cerebellum of a mouse model of Alzheimer disease. Methods Twelve 12-month-old APPswe/PS△E9
double transgenic mice and 12 wild-type C57 mice were sacrificed and the brain tissues were taken for examination. The right
hemisphere was stained with Congo red to observe the deposition of amyloid substances, and from the left hemisphere, the
hippocampus and the cerebellum were dissected for detecting the expression of miRNA-135a-5p, miRNA-298-5p,
miRNA-466b-3p and miR-669f-3p using real-time PCR. Results Congo red staining revealed the presence of Aβ deposition in
both the hippocampus and the cerebellum of the transgenic mice but not in the control mice. Real-time PCR showed a
significantly lower expression of the 4 miRNAs in the hippocampus in the transgenic mice than in the control mice (P<0.05).
The expression of miRNA-135a-5p, miRNA-298-5p, and miR-669f-3p in the cerebellum was significantly lower in the
transgenic mice than in the control mice (P<0.05). The expression of miRNA-298-5p and miR-669f-3p in the hippocampus was
significantly lower than that in the cerebellum of the transgenic mice (P<0.05). Conclusion Aβ deposition also occurs in the
cerebellum of APPswe/PS △ E9 double transgenic mice, and its formation might be related to the down-regulation of
miRNA-135a-5p, miRNA-298-5p, and miR-669f-3p.

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更新日期/Last Update: 1900-01-01