[1]朱国华,张琦,戴海萍,等.姜黄素诱导Jurkat细胞凋亡时MAPKs及MMPs家族的表达[J].南方医科大学学报,2013,(12):1792.
点击复制

姜黄素诱导Jurkat细胞凋亡时MAPKs及MMPs家族的表达()
分享到:

《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2013年12期
页码:
1792
栏目:
出版日期:
2013-12-15

文章信息/Info

Title:
Effect of curcumin on expressions of mitogen-activated protein kinases and matrix
metalloproteinases in Jurkat cells
作者:
朱国华张琦戴海萍沈群
关键词:
姜黄素Jurkat细胞胞内丝裂原活化蛋白激酶家族基质金属蛋白酶MAPKs
Keywords:
curcumin Jurkat cells mitogen-activated protein kinases matrix metalloproteinases MAPKs
摘要:
目的探讨姜黄素体外诱导人T细胞淋巴瘤Jurkat 细胞凋亡时MAPKs及MMPs家族成员的表达,揭示其可能的分子机
制。方法以不同浓度姜黄素作用Jurkat 细胞不同时间,MTT检测细胞增殖抑制率,流式细胞术检测细胞周期变化,Westernblot
检测MAPKs家族成员的表达,明胶酶谱法检测细胞培养上清中MMPs的活性。结果姜黄素呈时间-剂量依赖性抑制
Jurkat细胞增殖,使细胞阻滞于G0/G1期。以6.25、12.50及25.00 μmol/L姜黄素分别处理Jurkat细胞,胞内JNK和P-JNK的表达
均呈浓度依赖性上升(P<0.01),而ERK1/2及P38 MAPK的表达与阴性对照组相比无明显改变。另细胞培养上清中MMP-2及
MMP-9的活性在各药物处理组中也基本不变。结论姜黄素在(6.25~25.00)μmol/L浓度范围时,可体外诱导Jurkat细胞的凋
亡,阻滞细胞于G0/G1期。其分子机制可能与激活MAPKs家族中的JNk信号通路有关,而与MMPs家族成员的活性无关。
Abstract:
Objective To investigate the expressions of mitogen-activated protein kinases (MAPKs) and matrix
metalloproteinases (MMPs) in apoptotic human T cell lymphoma Jurkat cells induced by curcumin in vitro and explore the
possible molecular mechanisms of curcumin-induced apoptosis. Methods Jurkat cells were treated with different
concentrations of curcumin, and the cell proliferation and cell cycle changes were detected by MTT assay and flow cytometry,
respectively. Western blotting and gelatin zymography were employed to examine the protein expression levels of MAPKs and
MMPs activity in the exposed cells. Results Curcumin inhibited the proliferation of Jurkat cells in a time- and dose-dependent
manner, and caused cell cycle arrest in G0/G1 phase. Treatment of Jurkat cells with 25, 50, and 75 μmol/L curcumin resulted in a
concentration-dependent increase of JNK and p-JNK expressions (P<0.01) without significantly affecting the expressions of
ERK1/2 and P38 MAPK or the activity of MMP-2 and MMP-9. Conclusion Curcumin within the concentration range of
6.25-25.00 μmol/L can induce apoptosis and cell cycle arrest of Jurkat cells, the mechanism of which might involve the
activation of JNK pathway but not the MMPs.

相似文献/References:

[1]邹红云,马骊,姚新生,等.Jurkat细胞TCR基因重排对BV CDR3的影响[J].南方医科大学学报,2006,(07):939.
 ZOU Hong-Yun,MA Li,YAO Xin-sheng,et al.Effects of T cell receptor gene rearrangement on BV CDR3 in Jurkat cells[J].,2006,(12):939.
[2]许刚,黄文,张卫民,等.姜黄素和儿茶素联用对二甲基肼诱导的大鼠大肠癌变过程中环氧合酶2表达的影响[J].南方医科大学学报,2005,(01):48.
 XU Gang,HUANG Wen,ZHANG Wei-min,et al.Effects of combined use of curcumin and catechin on cyclooxygenase-2 mRNA expression in dimethylhydrazine-induced rat colon carcinogenesis[J].,2005,(12):48.
[3]邱实,谭升顺,张江安,等.姜黄素诱导人黑色素瘤A375细胞凋亡以及对c-myc、caspase-3表达的影响[J].南方医科大学学报,2005,(12):1517.
 QIU Shi,TAN Sheng-shun,ZHANG Jiang-an,et al.Apoptosis induced by curcumin and its effect on c-myc and caspase-3 expressions in human melanoma A375 cell line[J].,2005,(12):1517.
[4]简燕婷,王继德,麦国丰,等.姜黄素对模型大鼠肠粘膜炎症因子的调控[J].南方医科大学学报,2004,(12):1353.
 JIAN Yan-ting,WANG Ji-de,MAI Guo-feng,et al.Modulation of intestinal mucosal inflammatory factors by curcumin in rats with colitis[J].,2004,(12):1353.
[5]李牧,王丽,刘海莉,等.姜黄素通过降低一氧化氮合酶水平抑制宫颈癌HeLa细胞的侵袭和转移[J].南方医科大学学报,2013,(12):1752.

更新日期/Last Update: 1900-01-01