[1]张雪光,洪权,侯剀,等.高尿酸通过miR-155下调eNOS表达而导致内皮细胞功能障碍[J].南方医科大学学报,2013,(08):1141.
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高尿酸通过miR-155下调eNOS表达而导致内皮细胞功能障碍()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2013年08期
页码:
1141
栏目:
出版日期:
2013-08-01

文章信息/Info

Title:
High concentration uric acid regulates endothelial function via miR-155
作者:
张雪光洪权侯剀王远大吴镝陈香美
关键词:
高尿酸血症microRNA-155内皮细胞
Keywords:
hyperuricemia microRNA-155 endothelial cells
摘要:
目的高尿酸血症是心血管和肾脏疾病的独立风险因素,本研究拟从miR-155入手初步探索其在高尿酸导致内皮功能障
碍过程的作用机制。方法培养人脐静脉内皮细胞系HUVEC,以600 μmol/L的尿酸孵育24、48 h之后收集细胞及细胞上清液,
检测eNOS的蛋白表达及上清液的NO含量;预测并利用双荧光素酶系统验证miR-155的靶基因,随后利用荧光定量PCR检测
高尿酸环境中内皮细胞miR-155的表达情况,通过miR-155 inhibitor的转染检测它对内皮功能障碍的影响。结果600 μmol/L
的尿酸能诱导内皮细胞eNOS表达下降,NO分泌减少;microRNA在线分析软件及双荧光素酶报告实验提示eNOS的翻译水平
受miR-155直接调控;同时发现高尿酸刺激内皮细胞后miR-155的表达上调,而miR-155 inhibitor能抑制它的表达;内皮细胞转
染miR-155 inhibitor之后再进行高尿酸的孵育,eNOS的表达及NO的分泌水平与单独高尿酸孵育组相比均显著升高。结论高
尿酸可通过miR-155下调eNOS表达而导致内皮细胞功能障碍。
Abstract:
Objective To investigate high-concentration uric acid-induced endothelial dysfunction mediated by miR-155.
Methods Human umbilical vein endothelial cells (HUVECs) were incubated with 600 μmol/L uric acid for 24 and 48 h, and
eNOS expression and NO content in the cell culture were measured. The target genes of miR-155 were predicted using on-line
analysis software and validated by dual-luciferase system. Real-time PCR was used to detect the expression of miR-155 in
endothelial cells incubated with high-concentration uric acid. The effect of miR-155 on endothelial dysfunction was assessed
by transfection of its inhibitor into HUVECs. Results The expression of eNOS and NO secretion decreased obviously in
HUVECs incubated with 600 μmol/L uric acid. MicroRNA on-line analysis software and dual luciferase reporter experiments
suggested that the level of eNOS translation was directly regulated by miR-155. The expression of miR-155 in endothelial cells
was upregulated after stimulation with high-concentration uric acid, and was inhibited by transfection of miR-155 inhibitor.
Expression of eNOS and secretion of NO were elevated in endothelial cells transfected with miR-155 inhibitor after incubation
with high-concentration uric acid. Conclusion High-concentration uric acid can down-regulate eNOS expression via miR-155
to induce endothelial dysfunction.

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更新日期/Last Update: 1900-01-01