[1]梁芬芬,刘翠平,黎丽旋,等.胃泌素对大鼠小肠上皮1,25(OH)2D3膜相关快速反应类固醇结合蛋白的影响[J].南方医科大学学报,2013,(07):990.
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胃泌素对大鼠小肠上皮1,25(OH)2D3膜相关快速反应类固醇结合蛋白的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2013年07期
页码:
990
栏目:
出版日期:
2013-07-15

文章信息/Info

Title:
Effects of gastrin on rat intestinal epithelial 1,25(OH)2D3-membrane associated rapid response steroid binding protein
作者:
梁芬芬刘翠平黎丽旋郭瑜白岚
关键词:
125D3-MARRS结合蛋白胃泌素小肠钙磷吸收
Keywords:
125D3-MARRS binding proteins gastrin calcium and phosphate uptake intestines
摘要:
目的探讨胃泌素对大鼠小肠上皮1,25(OH)2D3膜相关快速反应类固醇结合蛋白(1,25D3-MARRS结合蛋白)表达的影响。
方法应用免疫组织化学、RT-PCR和免疫印迹法,检测胃泌素、奥美拉唑处理后的SD大鼠小肠中1,25D3-MARRS结合蛋白的表
达情况与对照组的差异,并测大鼠血清中钙、磷离子的浓度变化。结果大鼠小肠上皮细胞中1,25D3-MARRS结合蛋白分布于
胞核、胞浆和胞膜。胃泌素、奥美拉唑均引起大鼠小肠组织1,25D3-MARRS结合蛋白的mRNA和蛋白两个水平表达增高(P<
0.05),但其作为一个促小肠钙磷吸收的蛋白,无明显增高血清钙磷浓度的作用(P>0.05)。结论1,25D3-MARRS结合蛋白作为
一个广泛分布的多功能蛋白,受胃泌素的调节,在消化道相关疾病具有很高的研究价值及临床应用前景。
Abstract:
Objective To explore the effects of gastrin on the expression of 1,25(OH)2D3-membrane associated rapid response
steroid (1,25D3-MARRS) binding protein in rat intestinal epithelium. Methods SD rats received intraperitoneal injections of
gastrin, omeprazole or physiological saline. The protein expression of 1,25D3-MARRS binding protein in SD rat intestinal was
determined with Western blotting and immunohistochemistry, and its mRNA levels determined by RT-PCR. The serum
calcium and phosphate levels in the rats were also detected. Results Immunohistochemistry showed that 1,25D3-MARRS
binding protein was expressed mainly in the nuclei, cytoplasm and membrane of the intestinal epithelial cells. Both the protein
and mRNA expression levels of 1,25D3-MARRS binding protein were up-regulated after treatments with gastrin and
omeprazole (P<0.05), but the serum calcium and phosphate concentrations showed no obvious increase. Conclusion 1,
25D3-MARRS binding protein, which is widely expressed with versatile functionalities, is regulated by gastrin and shows high
potentials in the study of gastrointestinal diseases.

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更新日期/Last Update: 1900-01-01