[1]卜锐,邹建中,银丽,等.离体猪肝机械灌注模型的建立及其在高强度聚焦超声研究中的意义[J].南方医科大学学报,2012,(11):1564.
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离体猪肝机械灌注模型的建立及其在高强度聚焦超声研究中的意义()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2012年11期
页码:
1564
栏目:
出版日期:
2012-11-15

文章信息/Info

Title:
Establishment of an isolated porcine liver machine perfusion model for high-intensity focused ultrasound studies
作者:
卜锐邹建中银丽伍烽孟世和刘芳赵欣焦娇杨含
关键词:
离体猪肝机械灌注模型高强度聚焦超声
Keywords:
isolated porcine livermachine perfusion model high-intensity focused ultrasound
摘要:
目的探讨建立离体猪肝机械灌注模型的可行性及在高强度聚焦超声(HIFU)基础研究中的意义。方法对屠宰场获取的
21例猪肝以双血管(经门静脉和肝动脉)、自体血灌注液、体外循环机离体灌注4h,监测肝脏的颜色、质地、质量、血流动力学(流
量及压力)、彩色多普勒超声影像、胆汁分泌量、组织病理学变化以评价灌注效果。结果19例猪肝同时经门静脉和肝动脉插管
成功,2例肝动脉插管失败。灌注后4h肝脏质地柔软、血流动力学各参数稳定且保持在相对正常的生理范围内,灌注3h内胆汁
分泌量>3ml/h,组织病理学显示脏组织总体无形态结构的改变。结论离体猪肝机械灌注模型是稳定、可行的,能满足HIFU基
础研究中的实验要求。
Abstract:
ObjectiveTo explore the feasibility of establishing an isolated porcine liver machine perfusion model and assess its
value in high-intensity focused ultrasound studies. MethodsTwenty-one isolated porcine livers were perfused with
autologous blood for4h through dual vessels (portal vein and hepatic artery) cannulation using an extracorporeal circulation
machine under a subnormothermic perfusion condition. The perfusion model was assessed by monitoring the liver color,
texture, liver weight gain, hemodynamic parameters, color Doppler flow imaging, bile output and histopathology. Results
Nineteen isolated porcine livers were successfully cannulated with dual vessels, and failure of hepatic artery intubation
occurred in two porcine livers. After machine perfusion for4h, the isolated livers maintained a soft texture with stable
hemodynamic levels within relative normal physiological ranges. The bile output was more than3ml/h within the initial3h of
perfusion. Histopathological examination demonstrated no morphological or structural changes of the liver tissues.
ConclusionThe isolated porcine liver perfusion model is stable and feasible, and can be used for high-intensity focused
ultrasound studies.

相似文献/References:

[1]焦娇,伍烽,邹建中,等.不同脉冲高强度聚焦超声辐照方式损伤离体灌注猪肝的实验研究[J].南方医科大学学报,2013,(02):230.

更新日期/Last Update: 1900-01-01