[1]钟雷,谭洁,欧阳石,等.知母皂苷B对大鼠海马注射β-AP(25~35)致tau蛋白磷酸化的影响[J].南方医科大学学报,2006,(08):1106-1109.
 ZHONG Lei,TAN Jie,OUYANG Shi,et al.Effects of Saponin B from Anemarrhena asphodeloides Bunge on tau hyperphosphorylation induced by beta-amyloid peptide (25-35) in rats[J].Journal of Southern Medical University,2006,(08):1106-1109.
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知母皂苷B对大鼠海马注射β-AP(25~35)致tau蛋白磷酸化的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2006年08期
页码:
1106-1109
栏目:
论著·基础研究
出版日期:
2000-01-01

文章信息/Info

Title:
Effects of Saponin B from Anemarrhena asphodeloides Bunge on tau hyperphosphorylation induced by beta-amyloid peptide (25-35) in rats
作者:
钟雷; 谭洁; 欧阳石; 徐江平;
广州军区武汉总医院神经内科; 解放军第94医院内三科; 南方医科大学药学院药理学系; 南方医科大学药学院药理学系 湖北武汉430070南方医科大学药学院药理学系; 广东广州510515; 江西南昌330002;
Author(s):
ZHONG Lei12 TAN Jie2 OUYANG Shi1 XU Jiang-ping1 1Department of Neurology Wuhan General Hospital of Guangzhou Command Wuhan 430070 China; 2Department of Pharmacology Southern Medical University Guangzhou 510515 China; 3Third Department of Internal Medicine 94 Hospital of PLA Nanchang 330002 China
关键词:
知母皂苷B tau蛋白 P53 DKK β淀粉样肽
Keywords:
saponins Anemarrhena asphodeloides Bunge tau protein p53 DKK β-amyloid
分类号:
R285
摘要:
目的观察大鼠双侧海马注射β淀粉样肽[β-AP(25 ̄35)]后,海马内p53、Wnt途径抑制剂DKK和磷酸化tau蛋白表达变化,并用此动物模型观察知母皂苷B的作用。方法用RT-PCR法检测p53和DKKmRNA表达的变化,用免疫组化法检测磷酸化的tau蛋白。结果AD模型组海马内神经元的tau蛋白过度磷酸化,而p53和DKKmRNA的表达与假手术对照组相比也显著增多;而知母皂苷B给药治疗组均可使这种现象得到明显改善。结论知母皂苷B可通过抑制β-AP引起的p53及其后续的DKK的表达增加而有效改善AD大鼠脑内神经元的tau蛋白过度磷酸化。?
Abstract:
Objective To investigate the changes in the expressions of p53, DKK (the inhibitor of Wnt pathway) and phosphorylated tau in rat bilateral hippocampus after β-amyloid peptide (β-AP) (25-35) injection, and observe the effect of saponin B from Anemarrhena asphodeloides Bunge (SAaB) in this model. Methods After bilateral injection of β-AP (25-35) into the hippocampus of rats, RT-PCR was performed for observing the changes in p53 and DKK mRNA expressions and immunochemistry carried out to detect the changes in phosphorylated tau protein. Results RT-PCR showed increased p53 and DKK mRNA expression and immunochemistry revealed increased phosphorylated tau-positive cells in rat hippocampus after β-AP (25-35) injection, and administration of SAaB significantly ameliorated these changes. Conclusion SAaB can significantly ameliorate β-AP-induced tau hyperphosphorylation by inhibiting increased p53 and DKK mRNA expressions in response to β-AP injection.

备注/Memo

备注/Memo:
国家863计划新药博士基金资助项目(2003AA2Z3535)~~
更新日期/Last Update: 1900-01-01