[1]程良红,金士正,高素青,等.中国南北汉族人群HLA-A*02等位基因的分布差异[J].南方医科大学学报,2005,(03):321-324.
 CHENG Liang-hong,JIN Shi-zheng,GAO Su-qing,et al.Difference in HLA-A*02 allele distribution between Han populations in south and north China[J].,2005,(03):321-324.
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中国南北汉族人群HLA-A*02等位基因的分布差异()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2005年03期
页码:
321-324
栏目:
出版日期:
2005-03-01

文章信息/Info

Title:
Difference in HLA-A*02 allele distribution between Han populations in south and north China
作者:
程良红 金士正 高素青 李桢 邹红岩 王大明 吴国光
深圳市血液中心, 广东, 深圳, 518035
Author(s):
CHENG Liang-hong JIN Shi-zheng GAO Su-qing LI Zhen ZOU Hong-yan WANG Da-ming WU Guo-guang
Shenzhen Blood Center, Shenzhen 518035, China
关键词:
中国汉族人类白细胞抗原HLA-A*02等位基因
Keywords:
Han NationalityHLAHLA-A?02alleles
分类号:
Q37
摘要:
目的 研究中国南北汉族人群中HLA-A*02等位基因的分布并比较其差异性。方法 采用PCR-SBT方法,对随机抽取的经PCR-SSP方法确认为HLA-A*02阳性的208例南方和129例北方汉族骨髓志愿供者进行序列分型。结果 南、北汉族人两个群体中均检出具有不同基因频率的6种A*02等位基因(A*020101、A*0203、A*0205、A*0206、A*0207和A*0210)。南方汉族人中A*0207(37%)为优势等位基因,A*020101(31%)、A*0203(16%)和A*0206(14%)比较常见;而北方汉族人则以A*020101(48%)为优势等位基因,A*0206(21%)和A*0207(23%)比较常见。两个群体中的A*02等位基因总体分布以及A*020101、A*0203和A*0207的相对频率均存在显著性差异。在高、低分辨率两个水平上,南方和北方汉族A*02杂合度分别高于90%和80%,并且低分辨率A*02纯合子在高分辨率水平上表现高度多样性并呈现一定的分布规律。结论 HLA-A*02等位基因在中国南、北汉族人群中的分布呈现高度杂合和遗传多样性并具有显著性差异,HLA-A*020101、A*0203和A*0207可作为人类学研究中区分中国南北汉族人群的遗传标志。
Abstract:
Objective To investigate the distributions of HLA-A?02 alleles in Han populations and compare their difference between the south and north in China.Methods A total of 208 individuals from south China and 109 from north China were randomly selected from registered bone marrow donors in Chinese Han population, who were tested positive for HLA-A*02 alleles by PCR with sequence-specific primers (PCR-SSP). Genotyping of the alleles was performed using PCR-sequence-based typing (PCR-SBT).Results Six different HLA-A?02 alleles (A?020101, A?0203, A?0205, A?0206, A?0207 and A?0210) were identified in the two Chinese Han populations, of which A?0207(37%) was the predominant allele in southerners and A?020101(48%) in the northerners. A?020101(31%), A?0203(16%) and A?0206(14%) were common alleles in the southerners in comparison with ?#8727;0206(21%) and A?0207(23%) alleles in the northerners. The overall distribution of A?02 alleles and the frequencies of A?020101, A?0203 and A?0207 in the two populations differed significantly. The heterozygosity of A?02 in the southerners and northerners was higher than 90% and 80% at the high- and low-resolution levels, respectively, and the distribution of A?02 homozygote at low-resolution level in both populations showed diversity and regularity at high-resolution level.Conclusions HLA-A?02 alleles possess high heterogeneity and genetic diversity in Chinese Han population with significantly different distributions in the two populations. HLA-A?020101, A?0203 and A?0207 may serve as the genetic markers for dividing Chinese Han individuals into southerners and northerners in anthropological studies.

参考文献/References:

[1] http://www.ebi.ac.uk/imgt/HLA.
[2] 赵桐茂.人类血型遗传学[M].北京:科学出版社,1987.363-5.
[3] 吴国光,邓志辉,高素青,等.中国汉族骨髓供者HLA多态性的研究[J].中华血液学杂志,2004,25(8):474-8.Wu GG,Deng ZH,Gao SQ,et al. Study of HLA polymorphism in the Chinese Han bone marrow registry donors [J]. Chin J Hematol,2004,25(8):474-8.
[4] 孙振球,徐勇勇.医学统计学[M].北京:人民卫生出版社,2002.540.
[5] Pera C,Parodi AM,Ferrara GB. Sequence-based typing for HLA-A long & medium size PCR protocol [A]. In:Tilanus MGJ,Hansen JH,Hurley CK,eds. IHWG technical manual,genomic analysis of the human MHC:DNA-based typing for HLA alleles and linked polymorphisms 2000:Publication of the 13th International Histocompatibility Working Group [M]. Seattle:Fred Hutchinson Cancer Research Center,2000.
[6] Yan CX,Wang RL,Li JX,et al. HLA-A gene polymorphism defined by high-resolution sequence-based typing in 161 northern Chinese Han people [J]. Geno Prot Bioinfo,2003,1 (4):304-9.
[7] 孙筱放,孙逸平,麦卫阳,等.中国南北地区两个人群HLA-A座位DNA分型的比较研究[J].中华医学遗传学杂志,1999,16(2):70-3.Sun XF,Sun YP,Mei WY,et al. A comparative study of HLA-A locus in Northern and Southern Chinese by means of PCR/SSOP typing[J]. Chin J Med Genet,1999,16(2):70-3.
[8] 闫春霞,宋艳萍,赖淑萍,等.应用PCR-SSOP技术研究中国汉族、维吾尔族HLA-A基因座基因多态性[J].遗传学报,2002,29(5):384-9.Yan CX,Song YP,Lai SP,et al. Analysis of DNA polymorphism at HLA-A locus by PCR amplification with sequence specific oligonucleotide probe in Chinese Han and Uygur populations [J].Acta Genet Sin,2002,29(5):384-9.
[9] 田伟,李立新,郭实士,等.中国南方汉族群体HLA-A2基因亚型分析[J].中国免疫学杂志,2000,16(4):198-9.
[10] Middleton D,Hawkins BR,Williams F,et al. HLA class I allele distribution of a Hong Kong Chinese population based on highresolution PCR-SSOP typing[J]. Tissue Antigens,2004,63:555-61.
[11] MiddletonD,WilliamsF,MeenaghA,et al. Analysis ofthe distribution of HLA-A alleles in populations from five continents [J]. Hum Immunol,2000,61:1048-52.
[12] 梁晓华,陈玫,胡荣花,等.大连地区HLA-A2人群基因多态性分析[J].中国输血杂志(Chin J Blood Transfus),2000,13(1):18.
[13] 侯亚非,孙宗棠,Appella E,等.北方汉族人群HLA-A2亚型分布及p53的合成肽体外诱导CTL反应[J].中华微生物学和免疫学杂志,1999,19(1):47-50.Hou YF,Sun ZT,Appella E,et al. HLA-A2 subtype distribution among Beijing Chinese and its effect on CTL response in vitro to p53 protein based synthetic peptides loaded on dendritic cells [J].Chin J Microbiol Immunol,1999,19(1):47-50.
[14] Fan L,Chen D,Gou S,et al. 12th International Histocompatibility Workgroup anthropology regional report:Asia-China,HLA and disease [A]. Oxford University Press,1997,292-4.
[15] 翟宁,冯喆,张雁征,等.沈阳地区汉族HLA-A*02亚型等位基因分布[J].中华医学遗传学杂志(Chin J Med Genet),1999,16(5):344-5.
[16] 任素萍.HLA-A2分子生物学研究进展[J].国外医学·分子生物学分册(Foreign Med Sci·Mol Biol Section),1999,21(3):170-4.
[17] Michael B,Peter K. Genetic diversity of HLA-A2:evolutionary and functional significance[J]. Immunol Today,1996,17(4):165-70.
[18] Morishima Y,Tannka Y,Mari M,et al. HLA-A2 allele disparity and its effect to clinical outcome in hematopoietic cell transplantation (HCT) from unrelated donors [A]. In:Hansen JA,Dupont B,eds.HLA 2004:Immunobiology of the Human MHC. Proceedings of 13th International Histocompatibility Workgoup and Congress.Volume Ⅰ & Ⅱ [M].Seattle:IHWG Press,2004.
[19] Zareparsi S,James BS,Kaye JA,et al. HLA-A2 homozygosity but not heterozygosity is associated with Alzheimer disease [J].Neurology,2002,58:973-5.
[20] 袁义达,张诚.中国姓氏:群体遗传和人口分布[M].上海:华东师范大学出版社,2002.71-2.
[21] 肖露露,陈洪涛,叶欣,等.HLA多态性在广东汉族人群分布的特殊性[J].中华微生物学和免疫学杂志,1999,19(4):302-5.Xiao LL,Chen HT,Ye X,et al. Characterization and distribution of HLA polymorphism in the Guangdong Han population [J]. Chin J Microbiol Immuol,1999,19(4):302-5.

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备注/Memo

备注/Memo:
收稿日期:2004-9-18。
基金项目:深圳市科技计划项目(200404134)
作者简介:程良红(1975- ),男,1997年毕业于湖南医科大学,本科,主要从事免疫遗传学研究,电话:0755-81617569,E-mail:chenglh99@yahoo.com.cn
更新日期/Last Update: 1900-01-01