[1]韩慧霞,姚连生,王爽,等.人鼻咽癌不同部位组织相关基因表达谱的研究[J].南方医科大学学报,2004,(10):1126-1129.
 HAN Hui-xia,YAO Lian-sheng,WANG Shuang,et al.Gene expression profiles in different tissues of human nasopharyngeal carcinoma[J].,2004,(10):1126-1129.
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人鼻咽癌不同部位组织相关基因表达谱的研究()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2004年10期
页码:
1126-1129
栏目:
出版日期:
2004-10-01

文章信息/Info

Title:
Gene expression profiles in different tissues of human nasopharyngeal carcinoma
作者:
韩慧霞1 姚连生2 王爽1 韩为农3 姚开泰1
1. 南方医科大学病理教研室, 广东, 广州, 510515;
2. 南方医科大学南方医院耳鼻咽喉科, 广东, 广州, 510515;
3. 南方医科大学中南大学湘雅医学院肿瘤研究所, 湖南, 长沙, 410078
Author(s):
HAN Hui-xia1 YAO Lian-sheng2 WANG Shuang1 HAN Wei-nong3 YAO Kai-tai1
关键词:
鼻咽癌基因芯片基因表达
Keywords:
nasopharygeal carcinomagene chipgene expression
分类号:
R392.3;R739.6
摘要:
目的 筛选鼻咽癌癌变发生过程中的重要基因。方法 应用显微切割和荧光标记探针基因芯片杂交技术,检测鼻咽癌、癌周组织、癌旁组织及鼻咽炎症组织的基因差异表达,扫描仪扫描荧光芯片,图象处理软件分析结果。结果 在检测的3组标本中,存在大量差异表达基因,涉及基因包括信号与蛋白传递、癌基因与原癌基因、免疫相关基因、凋亡基因,以及DNA结合转录和转录因子等范围。结论 鼻咽癌癌变过程中有多个多种类基因参与,说明鼻咽癌癌变过程是一个复杂、多通路的过程。
Abstract:
Objective To screen the genes that may play an important role in the carcinogenesis of nasopharyngeal carcinoma (NPC).Methods Microdissection and cDNA genechip hybridization techniques were used to examine the differentially expressed genes in NPC tissue,the surrounding and adjacent tissues of NPC,and the nasopharyngeal inflammation tissue. The fluorescent signals on cDNA chip were scanned and the results of hybridization analyzed by image processing software. Results Many differentially expressed genes were identified between the three samples,including many different types of genes,such as those responsible for signal and protein transmission,oncogene and tumor suppression genes,immune-associated genes,apoptosis genes and DNA binding and transcription factor genes.Conclusion The carcinogenesis of NPC involves many genes of a variety of types,suggesting its complex process.

参考文献/References:

[1] 游绍进,姚开泰,曹亚,等.Epstein-Barr病毒潜伏状态与鼻咽癌的关系[J].中华肿瘤杂志,1996,18(1):23-6You SJ,Yao KT,Cao Y,et al.Latency of Epstein-Barr virus and its relationship to nasopharyngeal carcinoma [J].Chin J Oncol,1996,18(1):23-6.
[2] Pathmanathan R,Prasad U,Sadler R,et al.Clonal proliferations of cells infected with Epstein-Barr virus in preinvasive lesions related to nasopharyngeal carcinoma [J].N Engl J Med,1995,333(11):693-8.
[3] Yeung WM,Zong YS,Chiu CT,et al.Epstein-Barr virus carriage by nasopharyngeal carcinoma in situ[J].Int J Cancer,1993,53 (5):746-50.
[4] Shalon D,Smith SJ,Brown PO,et al.A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization [J].Genome Res,1996,6(7):639-45.
[5] Sehgal A,Boynton AL,Young RF,et al.Application of the differential hybridization of AtlasTM human expression arrays technique in the identification of differentially expressed genes in human glioblastoma multiform tumor tissue[J].J Surg Oncol,1998,67(4):234-41.
[6] Clark EA,Golub T,Lander E,et al.Genomic analysis of metastasis reveals an essential role for RhoC [J].Nature,2000,406(6795):532-5.
[7] Li ZK,Yao KT,Cao Y.Molecular cloning of a novel tissue-specific gene from human nasopharyngeal epithelium [J].Gene,1999,237(1):235-40.
[8] 曾亮,关新元,唐发清,等.鼻咽癌转移相关基因的比较基因组杂交和cDNA微阵列研究[J].中国生物化学与分子生物学报,2002,18(5):588-93.Zeng L,Guan XY,Tang FQ,et al.Matestasis associated gene in nasopharyngeal carcinoma by comparative genomic hybridization and cDNA array[J].Chin J Biochem and Mol Biol,2002,18(5):588-93.
[9] 庄建良,明德松,许荣誉.应用微矩阵表达谱基因芯片检测食管癌的基因改变[J].中华实验外科杂志,2002,19(6):527-8.Zhuang JL,Ming DS,Xu RY.Study on differentially expressed genes in esophageal cancer by cDNA microarray [J].Chin J Exp Surg,2002,19(6):527-8.
[10] 孙青,丁彦青,高雪芹,等.肿瘤转移相关基因cDNA芯片的制备与应用[J].第一军医大学学报,2002,22(12):1070-5Sun Q,Ding YQ,Gao XQ,et al.Development and application of cDNA microarray of tumor metastasis-associated genes [J].J First Mil Med Uni/Di Yi Jun Yi Da Xue Xue Bao,2002,22(12):1070-5.
[11] Leethanakul C,Patel V,Gillespie J,et al.Distinct pattern of expression of differentiation and growth-related genes in squamous cell carcinomas of the head and neck revealed by the use of laser capture microdissection and cDNA arrays [J].Oncogene,2000,19 (28):3220-4.
[12] Wright KO,Messing EM,Reeder JE.Increased expression of the acid sphingomyelinase-like protein ASML3a in bladder tumors [J].J Urol,2002,168(6):2645-9.
[13] Kumar D,Whiteside TL,Kasid U.Identification of a novel necrosis factor- alpha- inducible gene,SCC-S2,containing the consensus sequence of a death effector domain of fas-associated death domainlike interleukin- 1 beta-converting enzyme-inhibitory protein[J].J Biol Chem,2000,275(4):2973-8.
[14] Freije JP,Fueyo A,Uria J,et al.Human Zn-alpha 2-glycoprotein cDNA cloning and expression analysis in benign and malignant breast tissues [J].FEBS Lett,1991,290(1-2):247-9.

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备注/Memo

备注/Memo:
收稿日期:2004-6-15。
基金项目:国家自然科学基金面上项目(39970784);广东省科技攻关项目(2KM054015)
作者简介:韩慧霞(1962- ),女,博士,副教授,硕士生导师,电话:020-61648223,E-mail:hhxia@fimmu.com
更新日期/Last Update: 1900-01-01