[1]李西平,钱新华,姚英民,等.DNA聚丙烯酰胺凝胶电泳银染方法的选择[J].南方医科大学学报,2004,(09):1072-1074.
 LI Xi-ping,QIAN Xin-hua,YAO Ying-min,et al.Comparison of several silver staining methods for DNA polyacrylamide gel electrophoresis[J].,2004,(09):1072-1074.
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DNA聚丙烯酰胺凝胶电泳银染方法的选择()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2004年09期
页码:
1072-1074
栏目:
出版日期:
2004-09-01

文章信息/Info

Title:
Comparison of several silver staining methods for DNA polyacrylamide gel electrophoresis
作者:
李西平 钱新华 姚英民 刘志杰 刘阳
第一军医大学南方医院儿科, 广东, 广州, 510515
Author(s):
LI Xi-ping QIAN Xin-hua YAO Ying-min LIU Zhi-jie LIU Yang
Department of Pediatrics, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China
关键词:
DNA银染聚丙烯酰胺凝胶电泳
Keywords:
DNAsilver stainingpolyacrylamide gel electrophoresis
分类号:
Q329.34
摘要:
目的 比较4种聚丙烯酰胺凝胶银染方法,为初次使用者选择方法时提供参考。方法 用24 mer的引物作为上样样本,分别采用氨水法、低浓度硝酸银法、0.1%及0.2%硝酸银法染色。后2种配合不同的显色剂显色。结果 前2种未显色,0.1%及0.2%硝酸银可显色。结论 0.2%的硝酸银与氢氧化钠显色剂相结合具有最高的灵敏度;0.2%的硝酸银与碳酸钠显色剂相结合具有最佳的显像效果。
Abstract:
Objective To compare 4 silver staining methods for DNA detection in polyacrylamide gel electrophoresis. Methods After the electrophoresis was completed, the gels were stained separately by four different methods, namely using ammonia-silver-citric acid, low-concentration silver nitrate, 0.1% and 0.2% silver nitrate. Results DNA was detected only by staining with 0.1% and 0.2% silver nitrate. Conclusion 0.2% silver nitrate used along with sodium hydroxide shows the highest sensitivity in DNA detection, while its use with sodium carbonate produces the best quality of the image.

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备注/Memo

备注/Memo:
收稿日期:2003-5-12。
基金项目:国家自然科学基金(39770784)
作者简介:李西平(1969-),女,第一军医大学在读博士研究生,电话:020-61641929
更新日期/Last Update: 1900-01-01