[1]王顺官,李琳.蚌肉提取物LX01对肺癌细胞株A549的抑制作用[J].南方医科大学学报,2004,(08):917-920.
 WANG Shun-guan,LI Lin.Inhibition of river clam extract LX01 on human lung epithelial carcinoma cell line A549[J].Journal of Southern Medical University,2004,(08):917-920.
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蚌肉提取物LX01对肺癌细胞株A549的抑制作用()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2004年08期
页码:
917-920
栏目:
出版日期:
2004-08-01

文章信息/Info

Title:
Inhibition of river clam extract LX01 on human lung epithelial carcinoma cell line A549
作者:
王顺官1 李琳2
1. 广东省第二人民医院肿瘤一科, 广东, 广州, 510317;
2. 第一军医大学药理学教研室, 广东, 广州, 510515
Author(s):
WANG Shun-guan1 LI Lin2
1. Department of Oncology, Second People’s Hospital of Guangdong Province, Guangzhou 510317, China;
2. Department of Pharmacology, First Military Medical University, Guangzhou 510515, China
关键词:
蚌肉提取物肺癌细胞株A549白细胞
Keywords:
river clam extractlung cancer cell strain A549leukocyte
分类号:
R730.53
摘要:
目的 观察蚌肉提取物LX01对肺癌细胞株A549的抑制作用及对血液白细胞数目的影响。方法 MTT法检测不同浓度的LX01对肺癌细胞株A549细胞增殖的抑制率;A549细胞接种BALB/C裸鼠,观察给药后肿瘤的生长情况,高、中、低剂量LX01对荷瘤小鼠的生存时间、瘤重及血液白细胞数目的影响。结果 LX01对A549细胞的增殖有明显的抑制作用,IC50为56.85mg/L,与阴性对照组比差异有显著性(P<0.01);LX01能剂量依赖性的抑制A549接种瘤的生长;LX01中、高剂量可明显延长A549荷瘤小鼠生存时间,高剂量组荷瘤瘤重明显减轻,与阳性对照药环磷酰胺相似,明显高于阴性对照组(P<0.01);与5-FU治疗组相比,LX01各剂量组对A549荷瘤小鼠血白细胞数目无降低作用。结论 蚌肉提取物LX01能显著抑制肺癌细胞株A549接种BALB/c裸鼠后的生长,且对免疫系统无明显抑制作用。
Abstract:
Objective To explore the inhibition effect of river clam extract LX01 on human lung epithelial carcinoma (A549) in mice. Methods The inhibition ratio of LX01 on A549 was detected by MTT method. A549 was inoculated into BALB/c mice, and 2 days later, LX01 (50, 100, 200 mg/kg·d) were administrated for 15 days. Tumor weight, survival time and leukocyte count were measured. Results Tumor growth was markedly inhibited by LX01 in a dose-depenner. LX01 could significantly extend the survival time compared with the control group (P<0.01), but LX01 did not reduce leukocyte count of mice transplanted with A549. Conclusion LX01 could efficiently inhibit the growth of A549 transplanted into mice and maintain the normal immunological function.

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备注/Memo

备注/Memo:
收稿日期:2003-10-26。
基金项目:2001年广东省中医药现代化重大科技专项项目(20010305A)
作者简介:王顺官(1967-),男,1990年毕业于第一军医大学临床医学专业,硕士,主治医师
更新日期/Last Update: 1900-01-01