[1]赵庆平,邹飞,陈光忠,等.N-甲基-D-天门冬氨酸受体介导缺氧SD大鼠PO/AH区神经元Ca2+浓度的变化[J].南方医科大学学报,2004,(04):461-463,466.
 ZHAO Qing-ping,ZOU Fei,CHEN Guang-zhong,et al.Changes in Ca2+ concentration mediated by N-methyl-D-aspartate receptor in PO/AH neurons of anoxic SD rats[J].,2004,(04):461-463,466.
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N-甲基-D-天门冬氨酸受体介导缺氧SD大鼠PO/AH区神经元Ca2+浓度的变化()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2004年04期
页码:
461-463,466
栏目:
出版日期:
2004-04-01

文章信息/Info

Title:
Changes in Ca2+ concentration mediated by N-methyl-D-aspartate receptor in PO/AH neurons of anoxic SD rats
作者:
赵庆平1 邹飞2 陈光忠1 李铁林1
1. 第一军医大学珠江医院神经外科, 广东, 广州, 510282;
2. 第一军医大学热带军队卫生学系高温医学研究室, 广东, 广州, 510515
Author(s):
ZHAO Qing-ping1 ZOU Fei2 CHEN Guang-zhong1 LI Tie-lin1
1. Department of Neurosurgery, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China;
2. Department of High-temperature Medicine, First Military Medical University, Guangzhou 510515, China
关键词:
N-甲基-D-天门冬氨酸Ca2+缺氧神经元丙戌酸钠
Keywords:
N-methyl-D-aspartateCa2+anoxianeuronsvaproic acid
分类号:
R338
摘要:
目的 研究N-甲基-D-天门冬氨酸(NMDA)受体特性,探讨NMDA对缺氧大鼠PO/AH区神经元Ca2+浓度的影响。方法 给大鼠应用NMDA受体激动剂NMDA和拮抗剂丙戊酸,观察其在正常和缺氧条件下对PO/AH区神经细胞内Ca2+浓度的影响。结果 在正常条件下,细胞内Ca2+荧光比值为0.95,第40秒加入NMDA后[Ca2+]i的荧光强度迅速增高,25s后达到峰值2.054,并稳定在此水平,其升幅为(109±52)%,加入激动剂后30 s内达到峰值3.783,并持续稳定在此水平,加入NMDA后[Ca2+]i升高了(286±91)%;缺氧条件下神经元[Ca2+]i在加入丙戌酸后由平台向下的降幅为(103±45)%。结论 [Ca2+]i的升高主要是由于NMDA受体通道开放,细胞外游离Ca2+易化扩散入胞内所致,丙戊酸可有效地降低NMDA受体活性,从而使细胞内游离Ca2+浓度明显下降,具有保护神经元免受缺氧损伤的作用。
Abstract:
Objective To study the changes of Ca2+ concentration mediated by N-methyl-D-aspartate (NMDA) receptor in the neurons in the preoptic area/anterior hypothalamus (PO/AH) of anoxic SD rats by investigating the properties of NMDA receptor. Methods The effects of NMDA receptor agonist NMDA and antagonist vaproic acid (VPA) on the[Ca2+]i in PO/AH neurons were observed in SD rats with anoxia. Results Under normal condition, the fluorescencet ratio was 0.95, which increased obviously in response to treatment with NMDA at 40 s and reached the peak value, 2.054, after 25 s with an increment of (109±52)%. After the addition of the agonist, the peak value reached 3.783 in 30 s and maintained the high level. The concentration of Ca2+ increased by (286±91)% after the treatment with NMDA. While in the anoxia group, the concentration of Ca2+ decreased by (103±45)% after the addition of VPA. Conclusions The increase in the concentration of Ca2+ results predominantly from the opening of NMDA receptor channel which allows Ca2+ influx. VPA may decrease the activity of NMDA receptor to reduce the Ca2+ concentration for the protection of the neurons against anoxia.

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备注/Memo

备注/Memo:
收稿日期:2003-9-18。
作者简介:赵庆平(1964-),男,主治医师,在读博士,电话:020-61643263
更新日期/Last Update: 1900-01-01