[1]腊蕾,饶进军,吴曙光.抑癌基因p53诱导Wnt通路抑制因子sFRP的表达[J].南方医科大学学报,2004,(04):453-455.
 LA Lei,RAO Jin-jun,WU Shu-guang.Anti-oncogene p53-induced expression of secreted frizzled-related protein,a Wnt signaling inhibitor[J].Journal of Southern Medical University,2004,(04):453-455.
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抑癌基因p53诱导Wnt通路抑制因子sFRP的表达()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2004年04期
页码:
453-455
栏目:
出版日期:
2004-04-01

文章信息/Info

Title:
Anti-oncogene p53-induced expression of secreted frizzled-related protein,a Wnt signaling inhibitor
作者:
腊蕾1 饶进军2 吴曙光1
1. 第一军医大学药物研究所, 广东, 广州, 510515;
2. 第一军医大学分校药理教研室, 广东, 广州, 510315
Author(s):
LA Lei1 RAO Jin-jun2 WU Shu-guang1
1. Institute of Pharmaceutic Science, First Military Medical University, Guangzhou 510515, China;
2. Department of Pharmacology, Branch Campus of First Military Medical University, Guangzhou 510315, China
关键词:
p53基因受体分泌型卷曲相关蛋白Wnt通路
Keywords:
p53 receptorsecretfrizzled-related proteinWnt signaling pathway Wnt
分类号:
R394.6;Q756
摘要:
目的 研究p53对Wnt通路抑制因子sFRP表达的调节作用。方法 将携带p53基因的复制缺陷型腺病毒载体(Adp53)导入到p53缺失的人肝癌细胞株Hep3B中,以流式细胞术检测Adp53转基因情况,以RT-PCR技术检测p53对sFRP表达的调节作用。结果 sFRP mRNA水平在转染p53 20 h后即有明显升高,其中以32 h达最高水平,随后逐渐降低。量效关系研究表明在转染剂量为0.05、0.5、5、50 pfu/cell的Adp53 sFRP mRNA表达均有显著增高,尤以5 pfu/cell时表达水平最高。结论 p53能明显诱导Wnt通路抑制剂sFRP的mRNA表达。
Abstract:
Objective To investigate the effects of anti-oncogene p53 on the expression of secreted frizzled-related protein (sFRP), a Wnt pathway inhibitor. Methods Human hepatocarcinoma Hep3B cells were transfected with a replication-defective adenovirus encoding p53 (Adp53), and Adp53 transgene expression was measured by fluorescence-activated cell sorting (FACS) and sFRP mRNA expression detected by reverse transcriptional (RT)-PCR. Results sFRP mRNA expression in Hep3B cells was potentiated 20 h after the transfection with Adp53, reaching the peak level at 32 h. Dose-effect studies revealed that Adp53 at the doses of 0.5, 5 and 50 pfu/cell could promote the expression of sFRP mRNA, with the highest expression occurred with the dose of 5 pfu/cell. Conclusion Ectogenetic p53 promotes mRNA expression of Wnt signaling pathway inhibitor sFRP in Hep3B cells.

参考文献/References:

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[2] Morin PJ, Sparks AB, Koriinek V, et al. Activation of 3-catenin-Tcf signaling in colon cancer by mutations in β-catenin or APC[J].Science, 1997, 275(3): 1787-89.
[3] Bienz M, Clevers H. Linking colorectal cancer to wrnt signaling[J].Cell, 2000, 103(2): 311-20.
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[6] Bafico A, Gazit A, Pramila T, et al. Interaction of frizzled related protein (FRP)with Wnt ligands and the frizzled receptor suggests alternative mechanisms for FRP inhibition of Wnt signaling[J]. J Biol Chem, 1999, 274(23):16180-187.
[7] Rattner A, Hsieh JC, Smallwood PM, et al. A family of secreted proteins contains homology to the cystine-rich ligand-binding domain of frizzled receptors[J]. Proc Natl Aced Sci, 1997, 94(7):2859-63.
[8] Kawano Y, Kypta R. Secreted antagonist of the Wnt signaling pathway[J]. J Cell Sci, 2003, 116(13): 2627-34.
[9] Roth W,Wild-Bode C, Platten M, et al. Secreted frizzled-related proteins inhibit motility and promote growth of human malignant glioma cells[J]. Oncogene 2000, 19(37): 4210-20.
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备注/Memo

备注/Memo:
收稿日期:2003-9-18。
作者简介:腊蕾(1972-),女,现读于第一军医大免疫药理学学博士,药师,电话:020-61648167,E-mail:LaLei88@163.com
更新日期/Last Update: 1900-01-01